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Sci Rep. 2017 Jun 19;7(1):3788. doi: 10.1038/s41598-017-04028-6.

Utilization of a Wheat660K SNP array-derived high-density genetic map for high-resolution mapping of a major QTL for kernel number.

Cui F1,2,3, Zhang N1,4, Fan XL1,5, Zhang W6,7, Zhao CH2, Yang LJ8, Pan RQ1,4, Chen M1,4, Han J1,4, Zhao XQ3, Ji J1,3, Tong YP3, Zhang HX2, Jia JZ9, Zhao GY10, Li JM11,12.

Author information

1
Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, 050022, China.
2
Genetic Improvement Centre of Agricultural and Forest Crops, College of Agriculture, Ludong Unversity, Yan'tai, 264025, China.
3
State Key Laboratory of Plant Cell and Chromosomal Engineering, Chinese Academy of Sciences, Beijing, 100101, China.
4
University of Chinese Academy of Sciences, Beijing, 10049, China.
5
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.
6
Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, 050022, China. edithor@126.com.
7
State Key Laboratory of Plant Cell and Chromosomal Engineering, Chinese Academy of Sciences, Beijing, 100101, China. edithor@126.com.
8
Xinxiang Academy of Agricultural Sciences, Xinxiang, 453000, China.
9
Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
10
Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. zhaoguangyao@caas.cn.
11
Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, 050022, China. ljm@sjziam.ac.cn.
12
State Key Laboratory of Plant Cell and Chromosomal Engineering, Chinese Academy of Sciences, Beijing, 100101, China. ljm@sjziam.ac.cn.

Abstract

In crop plants, a high-density genetic linkage map is essential for both genetic and genomic researches. The complexity and the large size of wheat genome have hampered the acquisition of a high-resolution genetic map. In this study, we report a high-density genetic map based on an individual mapping population using the Affymetrix Wheat660K single-nucleotide polymorphism (SNP) array as a probe in hexaploid wheat. The resultant genetic map consisted of 119 566 loci spanning 4424.4 cM, and 119 001 of those loci were SNP markers. This genetic map showed good collinearity with the 90 K and 820 K consensus genetic maps and was also in accordance with the recently released wheat whole genome assembly. The high-density wheat genetic map will provide a major resource for future genetic and genomic research in wheat. Moreover, a comparative genomics analysis among gramineous plant genomes was conducted based on the high-density wheat genetic map, providing an overview of the structural relationships among theses gramineous plant genomes. A major stable quantitative trait locus (QTL) for kernel number per spike was characterized, providing a solid foundation for the future high-resolution mapping and map-based cloning of the targeted QTL.

PMID:
28630475
PMCID:
PMC5476560
DOI:
10.1038/s41598-017-04028-6
[Indexed for MEDLINE]
Free PMC Article

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