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J Cell Biol. 2017 Aug 7;216(8):2339-2354. doi: 10.1083/jcb.201512055. Epub 2017 Jun 19.

A bifurcated signaling cascade of NIMA-related kinases controls distinct kinesins in anaphase.

Author information

1
Department of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, Lebanon, NH.
2
Norris Cotton Cancer Center, Dartmouth-Hitchcock Medical Center, Lebanon, NH.
3
Department of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, Lebanon, NH scott.a.gerber@dartmouth.edu.
4
Department of Molecular and Systems Biology, Geisel School of Medicine at Dartmouth, Lebanon, NH.

Abstract

In mitosis, cells undergo a precisely orchestrated series of spatiotemporal changes in cytoskeletal structure to divide their genetic material. These changes are coordinated by a sophisticated network of protein-protein interactions and posttranslational modifications. In this study, we report a bifurcation in a signaling cascade of the NIMA-related kinases (Neks) Nek6, Nek7, and Nek9 that is required for the localization and function of two kinesins essential for cytokinesis, Mklp2 and Kif14. We demonstrate that a Nek9, Nek6, and Mklp2 signaling module controls the timely localization and bundling activity of Mklp2 at the anaphase central spindle. We further show that a separate Nek9, Nek7, and Kif14 signaling module is required for the recruitment of the Rho-interacting kinase citron to the anaphase midzone. Our findings uncover an anaphase-specific function for these effector kinesins that is controlled by specific Nek kinase signaling modules to properly coordinate cytokinesis.

PMID:
28630147
PMCID:
PMC5551695
DOI:
10.1083/jcb.201512055
[Indexed for MEDLINE]
Free PMC Article

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