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Sensors (Basel). 2017 Jun 19;17(6). pii: E1435. doi: 10.3390/s17061435.

Design and Performance of a Portable and Multichannel SPR Device.

Author information

1
Key Laboratory of Biorheological Science and Technology, Chongqing University, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China. zhangxiaoling@cqu.edu.cn.
2
Chongqing Engineering Research Center of Medical Electronics Technology, Chongqing University, Bioengineering College, Chongqing University, Chongqing 400030, China. 20141902049@cqu.edu.cn.
3
Key Laboratory of Biorheological Science and Technology, Chongqing University, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China. 20126461@cqu.edu.cn.
4
Chongqing Engineering Research Center of Medical Electronics Technology, Chongqing University, Bioengineering College, Chongqing University, Chongqing 400030, China. 20126461@cqu.edu.cn.
5
Key Laboratory of Biorheological Science and Technology, Chongqing University, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China. huning@cqu.edu.cn.
6
Chongqing Engineering Research Center of Medical Electronics Technology, Chongqing University, Bioengineering College, Chongqing University, Chongqing 400030, China. huning@cqu.edu.cn.
7
Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, China. zyang@tmmu.edu.cn.
8
Key Laboratory of Biorheological Science and Technology, Chongqing University, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China. 20121913019t@cqu.edu.cn.
9
Department of Biomedical Engineering, Chongqing Medical University, Chongqing 400016, China. wangzhenyu20090306@gmail.com.
10
Key Laboratory of Biorheological Science and Technology, Chongqing University, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China. bioyangjun@cqu.edu.cn.

Abstract

A portable multichannel surface plasmon resonance (SPR) biosensor device is presented in this study. As an optical biosensor device, the core component of its light path is a semi-cylindrical prism, which is used as the coupling unit for the excitation of the SPR phenomena. Based on this prism, a wedge-shaped incident light beam including a continuous angle range (10°) is chosen to replace the commonly-used parallel light beam in traditional SPR devices, in which the incident angle is adjusted by a sophisticated mechanical system. Thus, complicated, cumbersome, and costly mechanical structures can be avoided in this design. Furthermore, the selection of a small and high-stability semiconductor laser and matrix CCD detector as well as a microfluidic system aids in the realization of a miniaturized and multichannel device. Several different samples were used to test the performance of this new device. For ethanol with different concentrations, the sensing response was of good linear relativity with the concentration (Y = 3.17143X + 2.81518, R² = 0.97661). Mouse IgG and goat anti-mouse IgG were used as biological samples for immunological analysis, and BSA as the control group. Good specific recognition between mouse IgG and goat anti-mouse IgG has been achieved. The detection limit of antibody to antigen coated on the sensing surface was about 25 mg/L without surface modification.

KEYWORDS:

microfluidic; portability; sensor; surface plasmon resonance

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