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Res Vet Sci. 2017 Dec;115:301-306. doi: 10.1016/j.rvsc.2017.06.004. Epub 2017 Jun 12.

Serum antioxidant capacity and oxidative damage in clinical and subclinical canine ehrlichiosis.

Author information

1
Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30100, Espinardo, Murcia, Spain.
2
Department of Internal Medicine, Faculty of Veterinary Medicine, Uludag University, 16059 Bursa, Turkey.
3
Department of Plant Biology, Faculty of Biology, University of Murcia, 30100, Espinardo, Murcia, Spain.
4
Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30100, Espinardo, Murcia, Spain. Electronic address: jjceron@um.es.

Abstract

The objective of this study was to evaluate and compare the antioxidant response and the products of oxidative damage analysed by various assays in clinical and subclinical canine monocytic ehrlichiosis (CME). For this purpose, four assays to measure the total serum antioxidant capacity (TAC), such as the cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of plasma (FRAP), trolox equivalent antioxidant capacity (TEAC) using acidic medium (TEACA), and the TEAC using the horseradish peroxidase (TEACH) were used. In addition, the serum thiol concentrations were analysed. Reactive oxygen species (ROS), thiobarbituric acid reactive substances (TBARS), and ferrous oxidation-xylenol orange (FOX) were measured to determine the concentrations of free radical and the products of oxidative damage as result of the disease. All antioxidant markers were significantly lower in the dogs on clinical ehrlichiosis when compared with healthy dogs; however only the CUPRAC, FRAP and thiol were significantly lower in subclinical CME compared with healthy dogs. TBARS and FOX showed no significant differences between dogs with CME and healthy dogs; however, a significant increased ROS concentration was observed in dogs with clinical and subclinical CME when compared with healthy dogs. Results showed that in CME there is a state of oxidative stress with significant changes in markers of antioxidant defence and in concentrations of free radicals. However, the detection of these changes would depend of the assay used.

KEYWORDS:

CUPRAC; Ehrlichiosis; Fox; Frap; ROS; Thiol

PMID:
28628845
DOI:
10.1016/j.rvsc.2017.06.004
[Indexed for MEDLINE]

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