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Mol Imaging. 2017 Jan 1;16:1536012117712638. doi: 10.1177/1536012117712638.

In Vivo PET Imaging of the Activated Immune Environment in a Small Animal Model of Inflammatory Arthritis.

Author information

1
1 Department of Radiology and Biomedical Imaging, University of California-San Francisco, San Francisco, CA, USA.
2
2 Cellsight Technologies, Inc, San Francisco, CA, USA.

Abstract

BACKGROUND:

Evolving immune-mediated therapeutic strategies for rheumatoid arthritis (RA) may benefit from an improved understanding of the complex role that T-cell activation plays in RA. This study assessed the potential of fluorine-18-labeled 9-β-d-arabinofuranosylguanine ([18F]F-AraG) positron emission tomography (PET) imaging to report immune activation in vivo in an adjuvant-induced arthritis (AIA) small animal model.

METHODS:

Using positron emission tomography-computed tomography imaging, uptake of [18F]F-AraG in the paws of mice affected by arthritis at 6 (acute) and 20 (chronic) days following AIA induction in a single paw was assessed and compared to uptake in contralateral control paws. Fractions of T cells and B cells demonstrating markers of activation at the 2 time points were determined by flow cytometry.

RESULTS:

Differential uptake of [18F]F-AraG was demonstrated on imaging of the affected joint when compared to control at both acute and chronic time points with corresponding changes in markers of T-cell activation observed on flow cytometry.

CONCLUSION:

[18F]F-AraG may serve as an imaging biomarker of T-cell activation in inflammatory arthritis. Further development of this technique is warranted and could offer a tool to explore the temporal link between activated T cells and RA as well as to monitor immune-mediated therapies for RA in clinical trials.

KEYWORDS:

animal models of disease; molecular imaging of inflammation

PMID:
28625080
PMCID:
PMC5480631
DOI:
10.1177/1536012117712638
[Indexed for MEDLINE]
Free PMC Article

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