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J Microbiol Biotechnol. 2017 Aug 28;27(8):1441-1448. doi: 10.4014/jmb.1703.03012.

A Rapid and Efficient Screening Method for Antibacterial Compound-Producing Bacteria.

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Korea Institute of Ocean Science & Technology, Jeju Special Self-Governing Province 63349, Republic of Korea.
Department of Marine Biology, Korea University of Science and Technology, Jeju Special Self-Governing Province 63349, Republic of Korea.
College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea.


Antibacterial compounds are widely used in the treatment of human and animal diseases. The overuse of antibiotics has led to a rapid rise in the prevalence of drug-resistant bacteria, making the development of new antibacterial compounds essential. This study focused on developing a fast and easy method for identifying marine bacteria that produce antibiotic compounds. Eight randomly selected marine target bacterial species (Agrococcus terreus, Bacillus algicola, Mesoflavibacter zeaxanthinifaciens, Pseudoalteromonas flavipulchra, P. peptidolytica, P. piscicida, P. rubra, and Zunongwangia atlantica) were tested for production of antibacterial compounds against four strains of test bacteria (B. cereus, B. subtilis, Halomonas smyrnensis, and Vibrio alginolyticus). Colony picking was used as the primary screening method. Clear zones were observed around colonies of P. flavipulchra, P. peptidolytica, P. piscicida, and P. rubra tested against B. cereus, B. subtilis, and H. smyrnensis. The efficiency of colony scraping and broth culture methods for antimicrobial compound extraction was also compared using a disk diffusion assay. P. peptidolytica, P. piscicida, and P. rubra showed antagonistic activity against H. smyrnensis, B. cereus, and B. subtilis, respectively, only in the colony scraping method. Our results show that colony picking and colony scraping are effective, quick, and easy methods of screening for antibacterial compound-producing bacteria.


Colony picking; antibacterial compounds; broth culture; colony scraping; screening

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