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Science. 2017 Jun 16;356(6343). pii: eaal4713. doi: 10.1126/science.aal4713.

Tubular clathrin/AP-2 lattices pinch collagen fibers to support 3D cell migration.

Author information

1
Inserm U1170, Gustave Roussy Institute, Université Paris-Saclay, Villejuif, France. guillaume.montagnac@gustaveroussy.fr nadia.elkhatib@gustaveroussy.fr.
2
Inserm U1170, Gustave Roussy Institute, Université Paris-Saclay, Villejuif, France.
3
Institut Curie, UMR144, Université de Recherche Paris Sciences et Lettres, Centre Universitaire, Paris, France.
4
Inserm/Université Pierre et Marie Curie UMR S974, Institut de Myologie, Paris, France.

Abstract

Migrating cells often use focal adhesions in order to move. Focal adhesions are less prominent in cells migrating in three-dimensional (3D) as compared with 2D environments. We looked for alternative adhesion structures supporting cell migration. We analyzed the dynamics of clathrin-coated pits in cells migrating in a 3D environment of collagen fibers. Both topological cues and local engagement of integrins triggered the accumulation of clathrin-coated structures on fibers. Clathrin/adaptor protein 2 (AP-2) lattices pinched collagen fibers by adopting a tube-like morphology and regulated adhesion to fibers in an endocytosis-independent manner. During migration, tubular clathrin/AP-2 lattices stabilized cellular protrusions by providing anchoring points to collagen fibers. Thus, tubular clathrin/AP-2 lattices promote cell adhesion that, in coordination with focal adhesions, supports cell migration in 3D.

PMID:
28619886
DOI:
10.1126/science.aal4713
[Indexed for MEDLINE]

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