Format

Send to

Choose Destination
J Cell Biol. 2017 Aug 7;216(8):2409-2424. doi: 10.1083/jcb.201701041. Epub 2017 Jun 15.

Molecular model of fission yeast centrosome assembly determined by superresolution imaging.

Author information

1
Stowers Institute for Medical Research, Kansas City, MO.
2
Stowers Institute for Medical Research, Kansas City, MO slj@stowers.org.
3
Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS.

Abstract

Microtubule-organizing centers (MTOCs), known as centrosomes in animals and spindle pole bodies (SPBs) in fungi, are important for the faithful distribution of chromosomes between daughter cells during mitosis as well as for other cellular functions. The cytoplasmic duplication cycle and regulation of the Schizosaccharomyces pombe SPB is analogous to centrosomes, making it an ideal model to study MTOC assembly. Here, we use superresolution structured illumination microscopy with single-particle averaging to localize 14 S. pombe SPB components and regulators, determining both the relationship of proteins to each other within the SPB and how each protein is assembled into a new structure during SPB duplication. These data enabled us to build the first comprehensive molecular model of the S. pombe SPB, resulting in structural and functional insights not ascertained through investigations of individual subunits, including functional similarities between Ppc89 and the budding yeast SPB scaffold Spc42, distribution of Sad1 to a ring-like structure and multiple modes of Mto1 recruitment.

PMID:
28619713
PMCID:
PMC5551712
DOI:
10.1083/jcb.201701041
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center