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Phytochemistry. 2017 Sep;141:147-155. doi: 10.1016/j.phytochem.2017.06.001. Epub 2017 Jun 12.

Chemical constituents from the fruits of Ligustrum japonicum and their inhibitory effects on T cell activation.

Author information

1
College of Pharmacy, Drug Research and Development Center, Catholic University of Daegu, Gyeongbuk, 38430, Republic of Korea.
2
College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
3
College of Pharmacy, Drug Research and Development Center, Catholic University of Daegu, Gyeongbuk, 38430, Republic of Korea. Electronic address: bsmin@cu.ac.kr.

Abstract

A previously undescribed nor-dammarane, 3β,20,23-trihydroxy-24,25,26,27-tetranordammarane; three previously undescribed secoiridoid glycosides, ligujaponosides A-B, and iso-oleonuzhenide; and twenty three known compounds, were isolated from the fruits of Ligustrum japonicum Thunb. Their chemical structures were elucidated by extensive spectroscopic analyses, including 1D and 2D NMR, and HRMS. The isolated compounds were screened for immunosuppressive effects on T activated cells by evaluating interleukin-2 (IL-2) production. Among them, sesamin inhibited IL-2 production in Jurkat T cells with an IC50 value of 38 ± 2 μM. In addition, sesamin inhibited the phosphorylation of extracellular signal-regulated protein kinase (ERK), a member of the mitogen-activated protein kinase (MAPK) family, in phorbol 12-myristate 13-acetate (PMA)/A23187-stimulated T cells. Therefore, sesamin was demonstrated to inhibit T cell activation via regulation of MAPK phosphorylation pathway.

KEYWORDS:

Ligustrum japonicum; Oleaceae; Secoiridoid; Sesamin; T cell activation; Triterpenoid

PMID:
28618355
DOI:
10.1016/j.phytochem.2017.06.001
[Indexed for MEDLINE]

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