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J Comp Neurol. 2017 Oct 1;525(14):3072-3082. doi: 10.1002/cne.24263. Epub 2017 Jul 6.

Altered expression of IGF-I system in neurons of the inflamed spinal cord during acute experimental autoimmune encephalomyelitis.

Author information

1
Department of Molecular Medicine, Faculty of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
2
Department of Immunology, Faculty of Medicine, Tehran University of Medical, Sciences, Tehran, Iran.
3
Australian Regenerative Medicine Institute, Monash University, Melbourne, Victoria, Australia.

Abstract

There is growing evidence that the impaired IGF-I system contributes to neurodegeneration. In this study, we examined the spinal cords of the EAE, the animal model of multiple sclerosis, to see if the expression of the IGF-I system is altered. To induce EAE, C57/BL6 mice were immunized with the Hooke lab MOG kit, sacrificed at the peak of the disease and their spinal cords were examined for the immunoreactivities (ir) of the IGF-I, IGF binding protein-1 (IGFBP-1) and glycogen synthase kinase 3β (GSK3β), as one major downstream molecule in the IGF-I signaling. Although neurons in the non EAE spinal cords did not show the IGF-I immunoreactivity, they were numerously positive for the IGFBP-1. In the inflamed EAE spinal cord however, the patterns of expressions were reversed, that is, a significant increased number of IGF-I expressing neurons versus a reduced number of IGFBP-1 positive neurons. Moreover, while nearly all IGF-I-ir neurons expressed GSK3β, some expressed it more intensely. Considering our previous finding where we showed a significant reduced number of the inactive (phosphorylated) but not that of the total GSK3β expressing neurons in the EAE spinal cord, it is conceivable that the intense total GSK3β expression in the IGF-I-ir neurons belongs to the active form of GSK3β known to exert neuroinflammatory effects. We therefore suggest that the altered expression of the IGF-I system including GSK3β in spinal cord neurons might involve in pathophysiological events during the EAE.

KEYWORDS:

(RRID: AB_177521); (RRID:AB_309643); (RRID:AB_310183); (RRID:AB_647604); EAE; GSK3β; IGF binding protein-1; IGF-I; multiple sclerosis; spinal cord

PMID:
28617951
DOI:
10.1002/cne.24263
[Indexed for MEDLINE]

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