Send to

Choose Destination
ACS Chem Biol. 2017 Aug 18;12(8):1980-1985. doi: 10.1021/acschembio.7b00038. Epub 2017 Jun 27.

Imaging with Mass Spectrometry of Bacteria on the Exoskeleton of Fungus-Growing Ants.

Author information

Department of Chemistry, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.
Department of Bacteriology, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.
School of Pharmacy, University of Wisconsin-Madison , Madison, Wisconsin 53705, United States.


Mass spectrometry imaging is a powerful analytical technique for detecting and determining spatial distributions of molecules within a sample. Typically, mass spectrometry imaging is limited to the analysis of thin tissue sections taken from the middle of a sample. In this work, we present a mass spectrometry imaging method for the detection of compounds produced by bacteria on the outside surface of ant exoskeletons in response to pathogen exposure. Fungus-growing ants have a specialized mutualism with Pseudonocardia, a bacterium that lives on the ants' exoskeletons and helps protect their fungal garden food source from harmful pathogens. The developed method allows for visualization of bacterial-derived compounds on the ant exoskeleton. This method demonstrates the capability to detect compounds that are specifically localized to the bacterial patch on ant exoskeletons, shows good reproducibility across individual ants, and achieves accurate mass measurements within 5 ppm error when using a high-resolution, accurate-mass mass spectrometer.

[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for American Chemical Society Icon for PubMed Central
Loading ...
Support Center