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J Proteome Res. 2017 Aug 4;16(8):2975-2982. doi: 10.1021/acs.jproteome.7b00273. Epub 2017 Jun 29.

Impact of Low-Intensity Pulsed Ultrasound on Transcript and Metabolite Abundance in Saccharomyces cerevisiae.

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Department of Biochemistry, University of Alberta , Edmonton, Alberta T6G 2V4, Canada.
InnTech Alberta , Vegreville, Alberta T6N 1E4, Canada.
Department of Biology, University of British Columbia , Okanagan Campus, Kelowna, British Columbia V1V 1V7, Canada.
Department of Chemistry, University of Alberta , Edmonton, Alberta T6G 2V4, Canada.
Department of Biomedical Engineering, University of Alberta , Edmonton, Alberta T6G 2V4, Canada.
Department of Electrical and Computer Engineering, University of Alberta , Edmonton, Alberta T6G 2V4, Canada.


The interactions of ultrasound with biological materials are exploited for diagnostic, interventional, and therapeutic applications in humans and can improve productivity in industrial-scale generation of organic molecules such as biofuels, vaccines, and antibodies. Accordingly, there is great interest in better understanding the biological effects of ultrasound. We studied the impact of low-intensity pulsed ultrasound (LIPUS) on RNA expression and metabolism of S. cerevisiae. Although the transcript expression signature of LIPUS-treated cells does not differ significantly from that of untreated cells after 5 days, metabolomic profiling by chemical-isotopic-labeling-liquid-chromatography-mass-spectrometry suggests that LIPUS has an impact on the pathways of pyrimidine, proline, alanine, aspartate, glutamate, and arginine metabolism. Therefore, LIPUS triggers metabolic effects beyond reprogramming of the core pathways of carbon metabolism. Further characterization of metabolism will likely be important for elucidation of the biological effects of LIPUS.


Saccharomyces cerevisiae; low-intensity pulsed ultrasound; metabolomics; transcriptomics

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