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Sci Transl Med. 2017 Jun 14;9(394). pii: eaal4508. doi: 10.1126/scitranslmed.aal4508.

Donor pulmonary intravascular nonclassical monocytes recruit recipient neutrophils and mediate primary lung allograft dysfunction.

Author information

1
Department of Surgery, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.
2
Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.
3
Department of Cell and Molecular Biology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.
4
Department of Surgery, Washington University School of Medicine, Saint Louis, MO 63110, USA.
5
Department of Surgery, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA. abharat@nm.org.

Abstract

Primary graft dysfunction is the predominant driver of mortality and graft loss after lung transplantation. Recruitment of neutrophils as a result of ischemia-reperfusion injury is thought to cause primary graft dysfunction; however, the mechanisms that regulate neutrophil influx into the injured lung are incompletely understood. We found that donor-derived intravascular nonclassical monocytes (NCMs) are retained in human and murine donor lungs used in transplantation and can be visualized at sites of endothelial injury after reperfusion. When NCMs in the donor lungs were depleted, either pharmacologically or genetically, neutrophil influx and lung graft injury were attenuated in both allogeneic and syngeneic models. Similar protection was observed when the patrolling function of donor NCMs was impaired by deletion of the fractalkine receptor CX3CR1. Unbiased transcriptomic profiling revealed up-regulation of MyD88 pathway genes and a key neutrophil chemoattractant, CXCL2, in donor-derived NCMs after reperfusion. Reconstitution of NCM-depleted donor lungs with wild-type but not MyD88-deficient NCMs rescued neutrophil migration. Donor NCMs, through MyD88 signaling, were responsible for CXCL2 production in the allograft and neutralization of CXCL2 attenuated neutrophil influx. These findings suggest that therapies to deplete or inhibit NCMs in donor lung might ameliorate primary graft dysfunction with minimal toxicity to the recipient.

PMID:
28615357
PMCID:
PMC5568853
DOI:
10.1126/scitranslmed.aal4508
[Indexed for MEDLINE]
Free PMC Article

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