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Pharmaceuticals (Basel). 2017 Jun 13;10(2). pii: E53. doi: 10.3390/ph10020053.

In Vitro and In Vivo Studies of Alar-Nasal Cartilage Using Autologous Micro-Grafts: The Use of the Rigenera® Protocol in the Treatment of an Osteochondral Lesion of the Nose.

Author information

1
Department of Public Health, Experimental Medicine and Forensic, Human Anatomy Unit, University of Pavia, Pavia 27100, Italy. gabriele.ceccarelli@unipv.it.
2
Center for Health Technologies, University of Pavia, Pavia 27100, Italy. gabriele.ceccarelli@unipv.it.
3
Chief of Plastic and Reconstructive Surgery, Catholic University, Tirana 1005, Albania. pietrogentile2004@libero.it.
4
Plastic and Reconstructive Surgery Department, University of Rome "Tor Vergata", Rome 00173, Italy. pietrogentile2004@libero.it.
5
Santa Croce Hospital, Unit of Orthopedics and Traumatology of Chieri and Moncalieri, Turin 10024, Italy. dottmarcarelli@gmail.com.
6
Department of Public Health, Experimental Medicine and Forensic, Human Anatomy Unit, University of Pavia, Pavia 27100, Italy. martina.balli01@universitadipavia.it.
7
Center for Health Technologies, University of Pavia, Pavia 27100, Italy. martina.balli01@universitadipavia.it.
8
Department of Public Health, Experimental Medicine and Forensic, Human Anatomy Unit, University of Pavia, Pavia 27100, Italy. flavio.ronzoni@unipv.it.
9
Center for Health Technologies, University of Pavia, Pavia 27100, Italy. flavio.ronzoni@unipv.it.
10
Department of Public Health, Experimental Medicine and Forensic, Human Anatomy Unit, University of Pavia, Pavia 27100, Italy. laura.benedetti@unipv.it.
11
Center for Health Technologies, University of Pavia, Pavia 27100, Italy. laura.benedetti@unipv.it.
12
Department of Public Health, Experimental Medicine and Forensic, Human Anatomy Unit, University of Pavia, Pavia 27100, Italy. cusella@unipv.it.
13
Center for Health Technologies, University of Pavia, Pavia 27100, Italy. cusella@unipv.it.

Abstract

Cartilage defects represent a serious problem due to the poor regenerative properties of this tissue. Regarding the nose, nasal valve collapse is associated with nasal blockage and persistent airway obstruction associated with a significant drop in the quality of life for patients. In addition to surgical techniques, several cell-based tissue-engineering strategies are studied to improve cartilage support in the nasal wall, that is, to ameliorate wall insufficiency. Nevertheless, there are no congruent data available on the benefit for patients during the follow-up time. In this manuscript, we propose an innovative approach in the treatment of cartilage defects in the nose (nasal valve collapse) based on autologous micro-grafts obtained by mechanical disaggregation of a small portion of cartilage tissue (Rigenera® protocol). In particular, we first analyzed in vitro murine and human cartilage micro-grafts; secondly, we analyzed the clinical results of a patient with pinched nose deformity treated with autologous micro-grafts of chondrocytes obtained by Rigenera® protocol. The use of autologous micro-graft produced promising results in surgery treatment of cartilage injuries and could be safely and easily administrated to patients with cartilage tissue defects.

KEYWORDS:

Rigenera® protocol; autologous micro-grafts; chondrocytes; nasal valve collapse; tissue engineering approaches

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