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Methods Mol Biol. 2017;1640:159-172. doi: 10.1007/978-1-4939-7165-7_11.

Isolation and Characterization of ARGONAUTE Mutants in Chlamydomonas.

Author information

1
Science Department, Natural Science Cluster, Kochi University, Kochi, 780-8520, Japan. tomohito@kochi-u.ac.jp.

Abstract

Random insertional mutagenesis and subsequent reverse genetic screening allow the isolation of mutants of interest. Here I describe the protocol for generating a tag insertion line and subsequent PCR-based screening for ARGONAUTE mutants as an example of a reverse genetic screen for the unicellular green alga Chlamydomonas reinhardtii.

KEYWORDS:

ARGONAUTE; Chlamydomonas; Genomic DNA extraction; Mutagenesis; PCR; miRNA

PMID:
28608341
DOI:
10.1007/978-1-4939-7165-7_11
[Indexed for MEDLINE]

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