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Nutrition. 2017 Jul - Aug;39-40:15-19. doi: 10.1016/j.nut.2017.02.003. Epub 2017 Feb 27.

Milk basic protein increases ghrelin secretion and bone mineral density in rodents.

Author information

1
Milk Science Research Institute, Megmilk Snow Brand Co., Ltd., Kawagoe, Saitama, Japan.
2
Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University, Sakuraku, Saitama, Japan.
3
Milk Science Research Institute, Megmilk Snow Brand Co., Ltd., Kawagoe, Saitama, Japan; Research and Development Planning Department, Megmilk Snow Brand Co., Ltd., Shinjuku-ku, Tokyo, Japan.
4
Research and Development Planning Department, Megmilk Snow Brand Co., Ltd., Shinjuku-ku, Tokyo, Japan.
5
Area of Life-NanoBio, Division of Strategy Research, Graduate School of Science and Engineering, Saitama University, Sakura-ku, Saitama, Japan.
6
Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University, Sakuraku, Saitama, Japan. Electronic address: Isakata@mail.saitama-u.ac.jp.

Abstract

OBJECTIVES:

Milk basic protein (MBP), a mixture of proteins isolated from bovine milk, is known to increase bone formation. Ghrelin, a stomach-derived peptide hormone, also has been reported to stimulate osteoblast formation. The aim of this study was to determine whether MBP-induced bone formation is mediated via ghrelin.

METHODS:

MBP was chronically administered to mice in their drinking water for 3 wk, and body weight, water intake, and bone mineral density were measured. Additionally, plasma bone-specific alkaline phosphatase, tartrate-resistant acid phosphatase isoform 5b, and ghrelin concentrations were determined by enzyme-linked immunosorbent assay. To examine the direct effect of MBP on ghrelin secretion, gastric tissue culture and primary mucosal cells were stimulated by MBP.

RESULTS:

The in vivo study of young, growing mice showed that chronic MBP intake for 3 wk increased the plasma ghrelin concentration and bone mineral density of the hind limb tibia. In vitro studies using minced rat gastric mucosa tissues and primary murine isolated gastric mucosal cells revealed that MBP stimulated ghrelin release in a dose-dependent manner. Moreover, MBP-induced ghrelin secretion was partly inhibited by adrenergic blockers.

CONCLUSIONS:

These findings suggest a novel mechanism by which MBP directly acts on ghrelin secretion. Additionally, the elevated ghrelin level induced by MBP may act as a mediator for bone formation.

KEYWORDS:

Bone mineral density; Ghrelin; Milk basic protein; Secretion; β-adrenergic receptor

PMID:
28606565
DOI:
10.1016/j.nut.2017.02.003
[Indexed for MEDLINE]

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