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Carbohydr Polym. 2017 Sep 15;172:265-274. doi: 10.1016/j.carbpol.2017.05.051. Epub 2017 May 21.

Ternary polysaccharide complexes: Colloidal drug delivery systems stabilized in physiological media.

Author information

1
Ingénierie des Matériaux Polymères, UMR CNRS 5223, Université Claude Bernard Lyon 1, 15 Bd. André Latarjet, 69622 Villeurbanne Cedex, France.
2
Institut de Biologie et Chimie des Protéines UMR 5305, CNRS/Université de Lyon, France.
3
B-Cell Design, 98 Rue Charles Legendre, 87000 Limoges, France.
4
Groupe Immunité des Muqueuses et Agents Pathogènes, INSERM Centre d'Investigation Clinique en Vaccinologie 1408, Université de Lyon, 15 rue Ambroise Paré, 42023 Saint-Etienne Cedex 2, France.
5
Ingénierie des Matériaux Polymères, UMR CNRS 5223, Université Claude Bernard Lyon 1, 15 Bd. André Latarjet, 69622 Villeurbanne Cedex, France. Electronic address: Delair@univ-lyon1.fr.

Abstract

Chitosan-hyaluronan (HYA) polyelectrolyte complexes (PECs) were designed to maintain their colloidal stabilities in physiological ionic strength and pH, via a new concept of ternary complexes. This strategy relied on the formation of a binary PEC between chitosan and a strong polyacid, dextran sulphate (DS) or heparin (HEP), and further functionalization with HYA. The major parameter leading to stabilized colloids was a high ratio of the degrees of polymerization of chitosan versus the strong polyacid. The process afforded either positive or negative particles when HYA was used in default or in excess (vs. chitosan) for the functionalization of the binary complexes. The most stable formulations were loaded with an antiretroviral drug tenofovir (TF), and could be surface functionalized with targeting IgAs. In vitro, the cationic TF loaded ternary complexes exhibited an inhibition of infection of PBMCs by the HIV-1 virus, superior to the free drug.

KEYWORDS:

Chitosan; HIV infection inhibition; Hyaluronan; Stabilization; Ternary complexes

PMID:
28606534
DOI:
10.1016/j.carbpol.2017.05.051
[Indexed for MEDLINE]

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