Format

Send to

Choose Destination
BMC Pharmacol Toxicol. 2017 Jun 12;18(1):46. doi: 10.1186/s40360-017-0150-9.

Profound perturbation induced by triclosan exposure in mouse gut microbiome: a less resilient microbial community with elevated antibiotic and metal resistomes.

Author information

1
Department of Environmental Health Science, University of Georgia, Athens, GA, 30602, USA.
2
Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA.
3
Department of Population Health and Pathobiology, North Carolina State University, Raleigh, 27606, USA.
4
Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA. kunlu@unc.edu.

Abstract

BACKGROUND:

Environmental chemical-induced perturbations of gut microbiome are associated with a series of adverse health outcomes. The effects of triclosan on human health have been controversial in recent years. The purpose of this study is to investigate the functional impact of triclosan on the mouse gut microbiome and the link between triclosan exposure and resistomes in gut bacteria.

METHODS:

We combined 16S rRNA gene sequencing and shotgun metagenomics sequencing to examine the compositional and functional impact of triclosan exposure on the gut microbiota of C57BL/6 mice.

RESULTS:

16S rRNA sequencing results revealed that 13-week triclosan exposure in drinking water induced significant perturbations in mouse gut bacterial assemblages with distinct trajectories compared to controls. Metagenomics sequencing results indicated a remarkable enrichment of gut bacterial genes related to triclosan resistance, stress response, antibiotic resistance and heavy metal resistance.

CONCLUSIONS:

Triclosan exposure has a profound impact on the mouse gut microbiome by inducing perturbations at both compositional and functional levels. To our best knowledge, this is the first evidence regarding the functional alterations of gut microbiome induced by triclosan exposure, which may provide novel mechanistic insights into triclosan exposure and associated diseases.

KEYWORDS:

Antibiotic resistance; Gut microbiome; Metal resistance; Resistome; Triclosan; Triclosan resistance

PMID:
28606169
PMCID:
PMC5469155
DOI:
10.1186/s40360-017-0150-9
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for BioMed Central Icon for PubMed Central
Loading ...
Support Center