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Nat Cell Biol. 2017 Jul;19(7):787-798. doi: 10.1038/ncb3559. Epub 2017 Jun 12.

Dynamic subunit turnover in ESCRT-III assemblies is regulated by Vps4 to mediate membrane remodelling during cytokinesis.

Author information

1
Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter (VBC), AT-1030 Vienna, Austria.
2
Department of Biochemistry, University of Geneva, CH-1211 Geneva, Switzerland.
3
U1006 INSERM, Aix-Marseille Université, 13009 Marseille, France.
4
Experimental Center, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, D-01307 Dresden, Germany.
5
Max Planck Institute of Molecular Cell Biology and Genetics, D-01307 Dresden, Germany.
6
Swiss National Centre for Competence in Research Programme Chemical Biology, CH-1211 Geneva, Switzerland.
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Contributed equally

Abstract

The endosomal sorting complex required for transport (ESCRT)-III mediates membrane fission in fundamental cellular processes, including cytokinesis. ESCRT-III is thought to form persistent filaments that over time increase their curvature to constrict membranes. Unexpectedly, we found that ESCRT-III at the midbody of human cells rapidly turns over subunits with cytoplasmic pools while gradually forming larger assemblies. ESCRT-III turnover depended on the ATPase VPS4, which accumulated at the midbody simultaneously with ESCRT-III subunits, and was required for assembly of functional ESCRT-III structures. In vitro, the Vps2/Vps24 subunits of ESCRT-III formed side-by-side filaments with Snf7 and inhibited further polymerization, but the growth inhibition was alleviated by the addition of Vps4 and ATP. High-speed atomic force microscopy further revealed highly dynamic arrays of growing and shrinking ESCRT-III spirals in the presence of Vps4. Continuous ESCRT-III remodelling by subunit turnover might facilitate shape adaptions to variable membrane geometries, with broad implications for diverse cellular processes.

PMID:
28604678
PMCID:
PMC5493987
DOI:
10.1038/ncb3559
[Indexed for MEDLINE]
Free PMC Article

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