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Biomed Mater. 2017 Oct 3;12(6):065003. doi: 10.1088/1748-605X/aa78d0.

Chitosan-based hydrogels for developing a small-diameter vascular graft: in vitro and in vivo evaluation.

Author information

1
Université Bordeaux, INSERM U1026, Bioingénierie tissulaire, F-33000 Bordeaux, France. CHU de Bordeaux, Pôle Odontologie et Santé Buccale, F-33000 Bordeaux, France.

Abstract

AIMS:

Vascular grafts made of synthetic polymers perform poorly in small-diameter applications (cardiac and peripheral bypass). Chitosan is a biocompatible natural polymer that can provide a novel biological scaffold for tissue engineering development. The goal of this study was to demonstrate the biocompatibility of a novel chitosan preparation in vitro and in vivo, and to assess its potential as a scaffold for vascular applications.

METHODS AND RESULTS:

A series of experiments of increasing complexity, ranging from in vitro biocompatibility and hemocompatibility tests to in vivo studies in small and large animals (rats and sheep), was performed to provide a comprehensive analysis of chitosan hydrogels' biological properties. In vitro studies established that: (i) chitosan supported human endothelial progenitor cells adhesion, proliferation and resistance to physiological shear stress; (ii) chitosan did not activate platelets, the complement system, or the intrinsic coagulation pathway. In vivo results showed: (iii) no resorption of chitosan and no chronic inflammation at 60 days in a rat heterotopic implantation model (magnetic resonance imaging and histology); (iv) no flow obstruction (Doppler ultrasound) and no thrombus formation (histology and scanning electron microscopy) at 2 h after a carotid arteriotomy repair with chitosan patches in sheep. Finally, two chitosan tubes were implanted as carotid interposition grafts for 3 days in sheep showing that chitosan was strong enough to be sutured, to withstand arterial pressure, and no flow obstruction was observed through this short period.

CONCLUSION:

Chitosan-based hydrogels displayed promising in vitro biocompatibility and hemocompatibility properties as well as in vivo short-term performance.

PMID:
28604360
DOI:
10.1088/1748-605X/aa78d0
[Indexed for MEDLINE]

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