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Pak J Pharm Sci. 2017 Jan;30(1):43-48.

Anti-inflammatory effects and mechanisms of Hizikia fusiformis via multicellular signaling pathways in lipopolysaccharide-induced RAW 264.7 cells.

Author information

1
Department of Oriental Medicine Resources and Institute of Korean Medicine Industry, Mokpo National University, Jeonnam, Republic of Korea.
2
Department of Marine and Fisheries Resources, Mokpo National University, Jeonnam, Republic of Korea.
3
Department of Food Engineering, Mokpo National University, Jeonnam, Republic of Korea.
4
Department of Oriental Medicine Resources and Institute of Korean Medicine Industry, Mokpo National University, Jeonnam, Republic of Korea / Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, USA.

Abstract

In this study, we investigated the anti-inflammatory effects and mechanisms of Hizikia fusiformis (HF) extracts in lipopolysaccharide (LPS)-induced RAW 264.7 cells. We extracted HF using solvent and sub-critical water techniques. In results, HF extracts inhibited nitric oxide (NO) production in cell-free and LPS-stimulated RAW 264.7 cells. HF210 (extract prepared with sub critical water at 210oC) was most effective. The HF210 extract dose-dependently inhibited inducible nitric oxide synthase expression (iNOS) and nuclear factor kappa (NF-B) p65 translocation from cytosol to the nucleus. Furthermore, HF210 extract dose-dependently inhibited the phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK), Jun N-terminal kinase (JNK), and signal transducers and activators of transcription (STAT)-1in LPS-induced RAW 264.7 cells. Thus, our results suggest that anti-inflammatory effects of HF210 extract showed a noticeable distinction by regulation of multiple signaling pathways in LPS-induced RAW 264.7 cells.

PMID:
28603111
[Indexed for MEDLINE]

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