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Fitoterapia. 2017 Jul;120:194-198. doi: 10.1016/j.fitote.2017.06.007. Epub 2017 Jun 8.

Investigation of pharmacokinetic parameters of bakuchicin isolated from Psoralea corylifolia in mice.

Author information

1
BK21 Plus KNU Multi-Omics based Creative Drug Research Team, College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 41566, Republic of Korea.
2
College of Pharmacy, The Catholic University of Korea, 43 Jibong-ro, Wonmi-gu, Bucheon-si, Gyeonggi-do 14662, Republic of Korea.
3
College of Pharmacy, Keimyung University, Daegu 42601, Republic of Korea.
4
Department of Periodontology, School of Dentistry, Kyungpook National University, Daegu 41940, Republic of Korea. Electronic address: leejm@knu.ac.kr.
5
BK21 Plus KNU Multi-Omics based Creative Drug Research Team, College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 41566, Republic of Korea. Electronic address: sangkyu@knu.ac.kr.

Abstract

Bakuchicin is a furanocoumarin isolated from the seeds of Psoralea corylifolia, which is used in oriental medicine. However, limited information on the pharmacokinetics of bakuchicin is available and in addition, no determined method has been devised to quantify bakuchicin levels in the plasma. In the present study, we developed and validated a quantification method using liquid chromatography (LC) coupled with tandem mass spectrometry (LC-MS/MS), which was applied to a pharmacokinetic investigation in mouse plasma. LC was performed using an ACE 5 C18 column, and a mixture of acetonitrile and water containing 0.1% formic acid was used as the mobile phase at a flow rate of 220μL/min. Bakuchicin transition ions in multiple reaction-monitoring modes using positive ionization were observed at m/z 187.0 to m/z 131.0. Bakuchicin and the internal standard (reserpine) had retention times of 4.5 and 4.3min, respectively. Acceptable linearity (r2=0.996) was observed over the concentration range of 20-1000ng/mL, with a lower quantification limit of 20ng/mL in mouse plasma. This method was successfully applied to determine the pharmacokinetic parameters of bakuchicin in mouse plasma and showed that the bioavailability of bakuchicin was 58.3% at 5mg/kg oral administration.

KEYWORDS:

Bakuchicin; Bioavailability; Liquid chromatography-tandem mass spectrometry; Pharmacokinetics; Psoralea corylifolia

PMID:
28602940
DOI:
10.1016/j.fitote.2017.06.007
[Indexed for MEDLINE]

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