A comprehensive analysis and annotation of human normal urinary proteome

Sci Rep. 2017 Jun 8;7(1):3024. doi: 10.1038/s41598-017-03226-6.

Abstract

Biomarkers are measurable changes associated with the disease. Urine can reflect the changes of the body while blood is under control of the homeostatic mechanisms; thus, urine is considered an important source for early and sensitive disease biomarker discovery. A comprehensive profile of the urinary proteome will provide a basic understanding of urinary proteins. In this paper, we present an in-depth analysis of the urinary proteome based on different separation strategies, including direct one dimensional liquid chromatography-tandem mass spectrometry (LC/MS/MS), two dimensional LC/MS/MS, and gel-eluted liquid fraction entrapment electrophoresis/liquid-phase isoelectric focusing followed by two dimensional LC/MS/MS. A total of 6085 proteins were identified in healthy urine, of which 2001 were not reported in previous studies and the concentrations of 2571 proteins were estimated (spanning a magnitude of 106) with an intensity-based absolute quantification algorithm. The urinary proteins were annotated by their tissue distribution. Detailed information can be accessed at the "Human Urine Proteome Database" (www.urimarker.com/urine).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers / urine*
  • Chromatography, High Pressure Liquid
  • Chromatography, Liquid
  • Computational Biology* / methods
  • Databases, Protein
  • Humans
  • Molecular Sequence Annotation
  • Proteome*
  • Proteomics* / methods
  • Tandem Mass Spectrometry

Substances

  • Biomarkers
  • Proteome