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Sci Rep. 2017 Jun 8;7(1):3031. doi: 10.1038/s41598-017-02946-z.

Burkholderia pseudomallei in a lowland rice paddy: seasonal changes and influence of soil depth and physico-chemical properties.

Author information

1
Institut de la Francophonie pour la Médecine Tropicale, BP 9519, Vientiane, Lao People's Democratic Republic.
2
Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic.
3
iEES-Paris (IRD-Sorbonne Universités -UPMC-CNRS-INRA-UDD-UPEC), c/o Department of Agricultural Land Management (DALaM), Vientiane, Lao People's Democratic Republic.
4
Centre for Tropical Medicine & Global Health, University of Oxford, Oxford, UK.
5
iEES-Paris (IRD-Sorbonne Universités -UPMC-CNRS-INRA-UDD-UPEC), c/o Soils and Fertilizers Research Institute (SFRI), Hanoi, Vietnam.
6
International Water Management Institute (IWMI), Southeast Asia Regional Office, PO BOX 4199, Vientiane, Lao People's Democratic Republic.
7
Géosciences Environnement Toulouse - GET (IRD, Université de Toulouse, CNES, CNRS, UPS), 31400, Toulouse, France.
8
iEES-Paris (IRD-Sorbonne Universités -UPMC-CNRS-INRA-UDD-UPEC), Université Pierre et Marie-Curie, Paris, France.
9
Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic. david.d@tropmedres.ac.
10
Centre for Tropical Medicine & Global Health, University of Oxford, Oxford, UK. david.d@tropmedres.ac.
11
Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK. david.d@tropmedres.ac.

Abstract

Melioidosis, a severe infection with the environmental bacterium Burkholderia pseudomallei, is being recognised increasingly frequently. What determines its uneven distribution within endemic areas is poorly understood. We cultured soil from a rice field in Laos for B. pseudomallei at different depths on 4 occasions over a 13-month period. We also measured physical and chemical parameters in order to identify associated characteristics. Overall, 195 of 653 samples (29.7%) yielded B. pseudomallei. A higher prevalence of B. pseudomallei was found at soil depths greater than the 30 cm currently recommended for B. pseudomallei environmental sampling. B. pseudomallei was associated with a high soil water content and low total nitrogen, carbon and organic matter content. Our results suggested that a sampling grid of 25 five metre square quadrats (i.e. 25 × 25 m) should be sufficient to detect B. pseudomallei at a given location if samples are taken at a soil depth of at least 60 cm. However, culture of B. pseudomallei in environmental samples is difficult and liable to variation. Future studies should both rely on molecular approaches and address the micro-heterogeneity of soil when investigating physico-chemical associations with the presence of B. pseudomallei.

PMID:
28596557
PMCID:
PMC5465195
DOI:
10.1038/s41598-017-02946-z
[Indexed for MEDLINE]
Free PMC Article

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