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Pharmacogenomics. 2017 Jun;18(9):853-963. doi: 10.2217/pgs-2017-0041. Epub 2017 Jun 8.

Rapid and reliable screening of HLA-B*15:02 in four Chinese populations using single-tube multiplex real-time PCR assay.

Author information

1
The National Engineering Research Center for Miniaturized Detection Systems, College of Life Science, Northwest University, Xi'an, China.
2
Key Laboratory of Synthetic & Natural Function Molecular Chemistry of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an, China.

Abstract

AIM:

 HLA-B*15:02 screening should be performed to prevent antiepileptic drug induced severe cutaneous adverse reactions in populations of Asian origin. This study aimed to develop fast and reliable HLA-B*15:02 genotyping method and to investigate the distribution of HLA-B*15:02 in different Chinese ethnicities.

MATERIALS & METHODS:

A single-tube multiplex real-time PCR assay for HLA-B*15:02 genotyping was established by combining allele-specific primers with TaqMan probes.

RESULTS:

The HLA-B*15:02 genotyping results in Bouyei (n = 100) by the established assay were completely consistent with the corresponding PCR sequence-based typing findings. The percentage of HLA-B*15:02 carrier in Bouyei (19%; n = 100) was significantly higher than those of Han (1%; n = 100), Tibetan (0%; n = 100) and Uyghur (0%; n = 50) populations (p < 0.001).

CONCLUSION:

The novel method provides rapid, reliable and cost-effective detection of HLA-B*15:02 allele in clinical applications.

KEYWORDS:

HLA-B*15:02; carbamazepine; severe cutaneous adverse drug reactions

PMID:
28594280
DOI:
10.2217/pgs-2017-0041
[Indexed for MEDLINE]

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