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J Pediatr Endocrinol Metab. 2017 May 1;30(5):507-515. doi: 10.1515/jpem-2016-0435.

Copy number variations in "classical" obesity candidate genes are not frequently associated with severe early-onset obesity in children.

Author information

1
Pediatric Research Center, University Hospital for Children and Adolescents, Medical Faculty, University of Leipzig, Leipzig.
2
Leipzig University Medical Center, IFB AdiposityDiseases, University of Leipzig, Leipzig.
3
Institute of Biochemistry, Molecular Biochemistry, Medical Faculty, University of Leipzig, Leipzig.
4
Wellcome Trust Centre for Human Genetics, Nuffield Department of Medicine, University of Oxford, Oxford.
5
Institute of Human Genetics, Medical Faculty, University of Leipzig, Leipzig.

Abstract

BACKGROUND:

Obesity is genetically heterogeneous and highly heritable, although polymorphisms explain the phenotype in only a small proportion of obese children. We investigated the presence of copy number variations (CNVs) in "classical" genes known to be associated with (monogenic) early-onset obesity in children.

METHODS:

In 194 obese Caucasian children selected for early-onset and severe obesity from our obesity cohort we screened for deletions and/or duplications by multiplex ligation-dependent probe amplification reaction (MLPA). As we found one MLPA probe to interfere with a polymorphism in SIM1 we investigated its association with obesity and other phenotypic traits in our extended cohort of 2305 children.

RESULTS:

In the selected subset of most severely obese children, we did not find CNV with MLPA in POMC, LEP, LEPR, MC4R, MC3R or MC2R genes. However, one SIM1 probe located at exon 9 gave signals suggestive for SIM1 insufficiency in 52 patients. Polymerase chain reaction (PCR) analysis identified this as a false positive result due to interference with single nucleotide polymorphism (SNP) rs3734354/rs3734355. We, therefore, investigated for associations of this polymorphism with obesity and metabolic traits in our extended cohort. We found rs3734354/rs3734355 to be associated with body mass index-standard deviation score (BMI-SDS) (p = 0.003), but not with parameters of insulin metabolism, blood pressure or food intake.

CONCLUSIONS:

In our modest sample of severely obese children, we were unable to find CNVs in well-established monogenic obesity genes. Nevertheless, we found an association of rs3734354 in SIM1 with obesity of early-onset type in children, although not with obesity-related traits.

KEYWORDS:

SIM1; children; copy number variations; genetic variants; obesity

PMID:
28593922
DOI:
10.1515/jpem-2016-0435
[Indexed for MEDLINE]

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