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Inflamm Res. 2017 Oct;66(10):843-853. doi: 10.1007/s00011-017-1063-1. Epub 2017 Jun 7.

Pam2CSK4 and Pam3CSK4 induce iNOS expression via TBK1 and MyD88 molecules in mouse macrophage cell line RAW264.7.

Author information

1
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
2
Department of Oral Microbiology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
3
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand. pongsak.uta@mahidol.ac.th.

Abstract

OBJECTIVE:

The aim of this study was to investigate the involvement of TLR adaptor molecules, such as TRIF, MyD88, and TBK1 in the induction of iNOS and nitric oxide (NO) production in Pam2CSK4 and Pam3CSK4-treated mouse macrophages.

METHOD:

Mouse macrophage cell line (RAW264.7) was transfected with trif, myd88, and tbk1 siRNAs before stimulated with Pam2CSK4 and Pam3CSK4. The iNOS gene and protein expression were determined by RT-PCR and immunoblotting, respectively. The NO production was determined by Griess reaction assay.

RESULTS:

The results showed that the induction of iNOS expression and NO production by Pam2CSK4 and Pam3CSK4 were diminished in tbk1 and myd88-depleted mouse macrophages but not trif-depleted cells.

CONCLUSION:

These results suggested that the TBK1 and MyD88 molecules were essential for the induction of iNOS expression and NO production by both Pam2CSK4 and Pam3CSK4 via TLR2 signaling.

KEYWORDS:

MyD88; TBK1; TLR2 ligands; TRIF; iNOS

PMID:
28593434
DOI:
10.1007/s00011-017-1063-1
[Indexed for MEDLINE]

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