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Nucleic Acids Res. 2017 Aug 21;45(14):8493-8507. doi: 10.1093/nar/gkx502.

Visualization of conformational variability in the domains of long single-stranded RNA molecules.

Author information

1
Laboratory of Plasma Membrane and Nuclear Signaling, Kyoto University Graduate School of Biostudies, Yoshida-konoe, Sakyo-ku, Kyoto 606-8501, Japan.
2
Laboratory of Science Communication, Kyoto University Graduate School of Biostudies, Yoshida-konoe, Sakyo-ku, Kyoto 606-8501, Japan.
3
Institute of Biotechnology, National Taiwan University, 4F, No. 81, Chang-Xing St, Taipei 106, Taiwan.

Abstract

We demonstrate an application of atomic force microscopy (AFM) for the structural analysis of long single-stranded RNA (>1 kb), focusing on 28S ribosomal RNA (rRNA). Generally, optimization of the conditions required to obtain three-dimensional (3D) structures of long RNA molecules is a challenging or nearly impossible process. In this study, we overcome these limitations by developing a method using AFM imaging combined with automated, MATLAB-based image analysis algorithms for extracting information about the domain organization of single RNA molecules. We examined the 5 kb human 28S rRNA since it is the largest RNA molecule for which a 3D structure is available. As a proof of concept, we determined a domain structure that is in accordance with previously described secondary structural models. Importantly, we identified four additional small (200-300 nt), previously unreported domains present in these molecules. Moreover, the single-molecule nature of our method enabled us to report on the relative conformational variability of each domain structure identified, and inter-domain associations within subsets of molecules leading to molecular compaction, which may shed light on the process of how these molecules fold into the final tertiary structure.

PMID:
28591846
PMCID:
PMC5737216
DOI:
10.1093/nar/gkx502
[Indexed for MEDLINE]
Free PMC Article

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