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Methods Mol Biol. 2017;1623:105-112. doi: 10.1007/978-1-4939-7095-7_9.

Follicular Dendritic Cell Isolation and Loading of Immune Complexes.

Author information

1
Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA, 02115, USA.
2
Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA, 02115, USA. michael.carroll@childrens.harvard.edu.

Abstract

Follicular dendritic cells (FDCs) are stromal cells that are centrally located within B cell follicles of lymph nodes and other lymphoid organs such as the spleen. Due to their relative low abundance and difficulty to isolate, FDCs are still largely an enigma. Here we describe how to isolate FDCs for ex vivo cell culture, sorting by flow cytometry and how to load them in vivo or in vitro with immune complexes.

KEYWORDS:

Cell culture; Cell isolation; FACS; Follicular dendritic cell; Immune complex

PMID:
28589351
DOI:
10.1007/978-1-4939-7095-7_9
[Indexed for MEDLINE]

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