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Nat Commun. 2017 Jun 6;8:15708. doi: 10.1038/ncomms15708.

Crop wild relative populations of Beta vulgaris allow direct mapping of agronomically important genes.

Author information

1
Plant Breeding Institute, Kiel University, Am Botanischen Garten 1-9, Kiel 24118, Germany.
2
CeBiTec &Faculty of Biology, Bielefeld University, Universitätsstraße 25, Bielefeld 33615, Germany.
3
Max Planck Institute for Molecular Genetics, Ihnestraße 73, Berlin 14195, Germany.
4
Garvan Institute of Medical Research, 384 Victoria Street, Darlinghurst, Sydney NSW 2010, Australia.
5
Syngenta Seeds AB, Box 302, Landskrona 26123, Sweden.
6
Department of Biotechnology, University of Natural Resources and Life Sciences (BOKU), Muthgasse 18, 1190 Vienna, Austria.
7
Centre for Genomic Regulation (CRG), Carrer del Dr. Aiguader 88, Barcelona 08003, Spain.
8
Department of Phytopathology, Institute of Sugar Beet Research (IfZ), Holtenser Landstraße 77, Göttingen 37079, Germany.
9
SESVanderHave N.V., Industriepark, Tienen 3300, Belgium.
10
KWS SAAT SE, Grimsehlstraße 31, Einbeck 37555, Germany.
11
Strube Research GmbH &Co. KG, Hauptstraße 1, Söllingen 38387, Germany.
12
Federal Research Centre for Cultivated Plants (JKI), Erwin-Baur-Str. 27, Quedlinburg 06484, Germany.

Abstract

Rapid identification of agronomically important genes is of pivotal interest for crop breeding. One source of such genes are crop wild relative (CWR) populations. Here we used a CWR population of <200 wild beets (B. vulgaris ssp. maritima), sampled in their natural habitat, to identify the sugar beet (Beta vulgaris ssp. vulgaris) resistance gene Rz2 with a modified version of mapping-by-sequencing (MBS). For that, we generated a draft genome sequence of the wild beet. Our results show the importance of preserving CWR in situ and demonstrate the great potential of CWR for rapid discovery of causal genes relevant for crop improvement. The candidate gene for Rz2 was identified by MBS and subsequently corroborated via RNA interference (RNAi). Rz2 encodes a CC-NB-LRR protein. Access to the DNA sequence of Rz2 opens the path to improvement of resistance towards rhizomania not only by marker-assisted breeding but also by genome editing.

PMID:
28585529
PMCID:
PMC5467160
DOI:
10.1038/ncomms15708
[Indexed for MEDLINE]
Free PMC Article

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