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Artif Cells Nanomed Biotechnol. 2018 May;46(3):599-606. doi: 10.1080/21691401.2017.1332636. Epub 2017 Jun 6.

Biosynthesized gold and silver nanoparticles by aqueous fruit extract of Chaenomeles sinensis and screening of their biomedical activities.

Author information

1
a Department of Oriental Medicinal Biotechnology, College of Life Science , Kyung Hee University , Yongin-si , Gyeonggi-do , Republic of Korea.
2
b Graduate School of Biotechnology and Ginseng Bank, College of Life Science , Kyung Hee University , Yongin-si , Gyeonggi-do , Republic of Korea.

Abstract

The design of mild and non-toxic synthesis of metallic nanoparticles is a topical subject in the nanotechnology field. The objective of this present study is to screen the bioactivity of biosynthesized nanoparticles by aqueous fruit extract of Chaenomeles sinensis. The reducing and stabilizing ability of C. sinensis to fabricate gold (Cs-AuNps) and silver (Cs-AgNps) nanoparticles was confirmed by UV-visible (UV-Vis) spectroscopy at 562 nm and 477 nm, respectively. The field-emission transmission electron microscopy (FE-TEM) and X-ray diffraction analysis (XRD) verify the nano-scale morphology and crystallinity of Cs-AuNps (20-40 nm) and Cs-AgNps (5-20 nm). Furthermore, we evaluated the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging capacity, antimicrobial activity against Staphylococcus aureus and Escherichia coli and cytotoxicity against breast cancer cells. The results showed that Cs-AuNps (IC50: 725.93 μg/mL) displayed superior inhibitory activities on DPPH than Cs-AuNps. The biosynthesized Cs-AuNps successfully inhibited the growth of pathogenic bacteria S. aureus (ATCC 6538) and E. coli (BL21). The cytotoxic effect of Cs-AuNps and Cs-AgNps was evaluated in murine macrophage (RAW264.7) and human breast cancer cell lines (MCF7) by MTT assay. Thus, the present study explores the biomedical applications of gold and silver nanoparticles synthesized by C. sinensis.

KEYWORDS:

Chaenomeles sinensis; antimicrobial activity; antioxidant activity; gold nanoparticles; silver nanoparticles

PMID:
28583007
DOI:
10.1080/21691401.2017.1332636
[Indexed for MEDLINE]

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