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Fungal Genet Biol. 2017 Aug;105:16-27. doi: 10.1016/j.fgb.2017.05.001. Epub 2017 Jun 1.

Fluorescent markers of various organelles in the wheat pathogen Zymoseptoria tritici.

Author information

1
School of Biosciences, University of Exeter, Exeter EX4 4QD, UK.
2
School of Biosciences, University of Exeter, Exeter EX4 4QD, UK; University of Utrecht, Department of Biology, Padualaan 8, 3584 CH Utrecht, The Netherlands. Electronic address: G.Steinberg@exeter.ac.uk.

Abstract

Development of novel strategies to control fungal plant pathogens requires understanding of their cellular organisation and biology. Live cell imaging of fluorescent organelle markers has provided valuable insight into various aspects of their cell biology, including invasion strategies in plant pathogenic fungi. Here, we introduce a set of 17 vectors that encode fluorescent markers to visualize the plasma membrane, endoplasmic reticulum (ER), chromosomes, the actin cytoskeleton, peroxisomes and autophagosomes in the wheat pathogen Zymoseptoria tritici. We fused either enhanced green-fluorescent protein (eGFP) or a codon-optimised version of GFP (ZtGFP) to homologues of a plasma membrane-located Sso1-like syntaxin, an ER signalling and retention peptide, a histone H1 homologue, the LifeAct actin-binding peptide, a mitochondrial acetyl-CoA dehydrogenase, a peroxisomal import signal and a homologue of the ubiquitin-like autophagosomal protein Atg8. We expressed these markers in wildtype strain IPO323 and confirmed the specificity of these markers by counterstaining or physiological experiments. This new set of molecular tools will help understanding the cell biology of the wheat pathogen Z. tritici.

KEYWORDS:

Autophagosomes; Endoplasmic reticulum; F-actin; Nuclei; Peroxisome; Plasma membrane; Septoria tritici blotch in wheat

PMID:
28579390
PMCID:
PMC5536155
DOI:
10.1016/j.fgb.2017.05.001
[Indexed for MEDLINE]
Free PMC Article

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