Format

Send to

Choose Destination
Stem Cell Res. 2017 Jul;22:33-42. doi: 10.1016/j.scr.2017.05.003. Epub 2017 May 15.

Isolation, characterization, and differentiation of multipotent neural progenitor cells from human cerebrospinal fluid in fetal cystic myelomeningocele.

Author information

1
Bioengineering, Cell Therapy and Surgery in Congenital Malformations Laboratory, Vall d'Hebron Research Institute (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain; Translational Research in Fetal Surgery for Congenital Malformations Laboratory, The Center for Fetal, Cellular, and Molecular Therapy, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
2
Bioengineering, Cell Therapy and Surgery in Congenital Malformations Laboratory, Vall d'Hebron Research Institute (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain.
3
Maternal-Fetal Medicine Unit, Department of Obstetrics, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.
4
Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, Baylor College of Medicine and Texas Children's Hospital, Houston, TX, USA.
5
Department of the Mother and Child Health, Pediatric Surgery Unit, Fondazione IRCCS Policlinico San Matteo, Pavia and University of Pavia, Italy.
6
Bioengineering, Cell Therapy and Surgery in Congenital Malformations Laboratory, Vall d'Hebron Research Institute (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain; Translational Research in Fetal Surgery for Congenital Malformations Laboratory, The Center for Fetal, Cellular, and Molecular Therapy, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA. Electronic address: Jose.Peiro@cchmc.org.

Abstract

Despite benefits of prenatal in utero repair of myelomeningocele, a severe type of spina bifida aperta, many of these patients will still suffer mild to severe impairment. One potential source of stem cells for new regenerative medicine-based therapeutic approaches for spinal cord injury repair is neural progenitor cells (NPCs) in cerebrospinal fluid (CSF). To this aim, we extracted CSF from the cyst surrounding the exposed neural placode during the surgical repair of myelomeningocele in 6 fetuses (20 to 26weeks of gestation). In primary cultured CSF-derived cells, neurogenic properties were confirmed by in vitro differentiation into various neural lineage cell types, and NPC markers expression (TBR2, CD15, SOX2) were detected by immunofluorescence and RT-PCR analysis. Differentiation into three neural lineages was corroborated by arbitrary differentiation (depletion of growths factors) or explicit differentiation as neuronal, astrocyte, or oligodendrocyte cell types using specific induction mediums. Differentiated cells showed the specific expression of neural differentiation markers (βIII-tubulin, GFAP, CNPase, oligo-O1). In myelomeningocele patients, CSF-derived cells could become a potential source of NPCs with neurogenic capacity. Our findings support the development of innovative stem-cell-based therapeutics by autologous transplantation of CSF-derived NPCs in damaged spinal cords, such as myelomeningocele, thus promoting neural tissue regeneration in fetuses.

KEYWORDS:

Cerebrospinal fluid; Fetal therapy; Human primary cell cultures; Myelomeningocele; Neural differentiation; Neural precursor cells

PMID:
28578005
DOI:
10.1016/j.scr.2017.05.003
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center