Adult albino zebrafish retinas were injected and electroporated with either standard control morpholino (SC MO) or anti-sox2 morpholino (sox2 MO) immediately prior to light treatment. Following the standard four days of constant light, fish were returned to a normal light/dark cycle to recover. After 21 days of recovery, retinal sections were prepared and labeled with photoreceptor markers Zpr1 (A–B, green), Rhodopsin (A–B, red), UV opsin (C–D, red), and Blue opsin (E–F, red). Fewer cones of all classes were observed in sox2 morphant retinas (B, D, F) compared to controls (A, C, E). Cones were often missing in small patches throughout the sox2 morphant retinas (B, asterisk). Rhodopsin labeled rod outer segments (ROS) appeared to be slightly shorter in length in sox2 morphant retinas (B) compared to controls (A). Quantification of photoreceptor markers (G) indicated a significant reduction in the numbers of cells expressing Zpr1 (Student’s t-test, p = 0.009, n = 10), Blue opsin (Student’s t-test, p = 0.003, n ≥ 8), and UV opsin (Student’s t-test, p = 8.37 × 10−6, n = 8), and cone nuclei (Student’s t-test, p = 0.007, n = 10) in sox2 morphants relative to control retinas. While the average thickness of the ONL was significantly reduced in sox2 morphant retinas compared to controls (H; Student’s t-test, p = 0.0127, n = 10), the length of ROS (H; Student’s t-test, p = 0.136, n = 10) and number of rod nuclei per 100 μm (G; Student’s t-test, p = 0.074, n = 10) was not statistically altered between groups. Retinas labeled with antibodies to PCNA (I–L, arrows), displayed a significant increase in ONL proliferation (M; Student’s t-test, p = 0.011, n = 10) in sox2 morphant retinas compared to controls. Retinas were stained with DAPI to detect the nuclear layers (A–F, K, L). PCNA, proliferating cell nuclear antigen; GCL, ganglion cell layer; INL, inn and I are 25 μm and are the same for panels B–F and J–L.