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Mater Sci Eng C Mater Biol Appl. 2017 Sep 1;78:748-755. doi: 10.1016/j.msec.2017.03.042. Epub 2017 Mar 6.

Fabrication of high-capacity polyelectrolyte brush-grafted porous AAO-silica composite membrane via RAFT polymerization.

Author information

1
Department of Materials Science and Engineering, College of Materials, Xiamen University, Xiamen 361005, China; Fujian Provincial Key Laboratory of Fire Retardant Materials, Xiamen University, Xiamen 361005, China.
2
Department of Materials Science and Engineering, College of Materials, Xiamen University, Xiamen 361005, China.
3
Xiamen Stomatology Hospital, Fujian Medical University, Xiamen 361003, China.
4
Xiamen Stomatology Hospital, Fujian Medical University, Xiamen 361003, China; Hospital of Stomatology, Fourth Military Medical University, Xian 710032, China. Electronic address: tinachen0628@163.com.
5
Department of Materials Science and Engineering, College of Materials, Xiamen University, Xiamen 361005, China; Fujian Provincial Key Laboratory of Fire Retardant Materials, Xiamen University, Xiamen 361005, China. Electronic address: lzdai@xmu.edu.cn.

Abstract

Surface-initiated reversible addition-fragmentation chain transfer (RAFT) polymerization has been utilized to fabricate high-capacity strong anion-exchange (AEX) membrane for the separation of protein. By means of RAFT polymerization, quaternized poly(3-(methacrylamidomethyl)-pyridine) brushes formed 3-dimensional nanolayers on the surface of porous anodic aluminum oxide (AAO)-silica composite membrane. The surface properties of the membranes were analyzed by SEM, water contact angle, ATR-FTIR, XPS and TGA. To investigate the adsorption performance, the new AEX membranes were applied to recover a model protein, ovalbumin (OVA). High adsorption capacities of 95.8mg/g membranes (static) and 65.3mg/g membranes (dynamic) were obtained at ambient temperature. In the further studies, up to 90% of the adsorbed OVA was efficiently eluted by using phosphate buffer-1M NaCl as elution medium. The successful separation of OVA with high purity from a mixture protein solution was also achieved by using the AEX membranes. The present study demonstrated that under mild reaction condition, RAFT polymerization can be used to fabricate ion-exchange membrane which has many remarkable features, such as high capacity and selectivity, easy elution and so on.

KEYWORDS:

AAO membrane; Ion-exchange membrane; Polyelectrolyte brush; Protein separation; RAFT

PMID:
28576045
DOI:
10.1016/j.msec.2017.03.042
[Indexed for MEDLINE]

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