Gold nanoparticles embedded electropolymerized thin film of pyrimidine derivative on glassy carbon electrode for highly sensitive detection of l-cysteine

Mater Sci Eng C Mater Biol Appl. 2017 Sep 1:78:513-519. doi: 10.1016/j.msec.2017.04.105. Epub 2017 Apr 19.

Abstract

This paper demonstrates the fabrication of novel gold nanoparticles incorporated poly (4-amino-6-hydroxy-2-mercaptopyrimidine) (Nano-Au/Poly-AHMP) film modified glassy carbon electrode and it is employed for highly sensitive detection of l-cysteine (CYS). The modified electrode was characterized by scanning electron microscope (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). SEM images of modified electrode revealed the homogeneous distribution of gold nanoparticles on poly (4-amino-6-hydroxy-2-mercaptopyrimidine) thin film modified glassy carbon electrode. The modified electrode was successfully utilized for highly selective and sensitive determination of l-cysteine at physiological pH7.0. The present electrochemical sensor successfully resolved the voltammetric signals of ascorbic acid (AA) and l-cysteine with peak separation of 0.510V. To the best of our knowledge, this is the first report of larger peak separation between AA and CYS. Wide linear concentration ranges (2μM-500μM), low detection limit (0.020μM), an excellent reproducibility and stability are achieved for cysteine sensing with this Nano-Au/Poly-AHMP/GCE.

Keywords: Electrocatalysis; Gold nanoparticles; Poly(4-amino-6-hydroxy-2-mercaptopyrimidine); l-cysteine.

MeSH terms

  • Carbon
  • Cysteine
  • Electrodes
  • Gold
  • Metal Nanoparticles*
  • Pyrimidines
  • Reproducibility of Results

Substances

  • Pyrimidines
  • Carbon
  • Gold
  • Cysteine