Format

Send to

Choose Destination
Nucleic Acids Res. 2017 Jul 7;45(12):7494-7506. doi: 10.1093/nar/gkx362.

Structural determinants of APOBEC3B non-catalytic domain for molecular assembly and catalytic regulation.

Author information

1
Genetic, Molecular and Cellular Biology Program, Keck School of Medicine, University of Southern California, Los Angeles, CA 90089, USA.
2
Molecular and Computational Biology, Departments of Biological Sciences and Chemistry, University of Southern California, Los Angeles, CA 90089, USA.
3
Department of Clinical Microbiology and Immunology of Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
4
161 Hospital, Wuhan 430012, China.
5
Polytech' Clermont-Ferrand, Université Blaise Pascal, Clermont-Ferrand, France.
6
Center of Excellence in NanoBiophysics, University of Southern California, Los Angeles, CA 90089, USA.
7
Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA 90089, USA.

Abstract

The catalytic activity of human cytidine deaminase APOBEC3B (A3B) has been correlated with kataegic mutational patterns within multiple cancer types. The molecular basis of how the N-terminal non-catalytic CD1 regulates the catalytic activity and consequently, biological function of A3B remains relatively unknown. Here, we report the crystal structure of a soluble human A3B-CD1 variant and delineate several structural elements of CD1 involved in molecular assembly, nucleic acid interactions and catalytic regulation of A3B. We show that (i) A3B expressed in human cells exists in hypoactive high-molecular-weight (HMW) complexes, which can be activated without apparent dissociation into low-molecular-weight (LMW) species after RNase A treatment. (ii) Multiple surface hydrophobic residues of CD1 mediate the HMW complex assembly and affect the catalytic activity, including one tryptophan residue W127 that likely acts through regulating nucleic acid binding. (iii) One of the highly positively charged surfaces on CD1 is involved in RNA-dependent attenuation of A3B catalysis. (iv) Surface hydrophobic residues of CD1 are involved in heterogeneous nuclear ribonucleoproteins (hnRNPs) binding to A3B. The structural and biochemical insights described here suggest that unique structural features on CD1 regulate the molecular assembly and catalytic activity of A3B through distinct mechanisms.

PMID:
28575276
PMCID:
PMC5499559
DOI:
10.1093/nar/gkx362
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center