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Methods Mol Biol. 2017;1621:21-27. doi: 10.1007/978-1-4939-7063-6_2.

Expression of Plant Receptor Kinases in Yeast.

Author information

1
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, "Dr. Héctor Torres" (INGEBI-CONICET), Vueltade Obligado 2490, C1428ADN, Buenos Aires, Argentina.
2
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, "Dr. Héctor Torres" (INGEBI-CONICET), Vueltade Obligado 2490, C1428ADN, Buenos Aires, Argentina. prometeo@dna.uba.ar.

Abstract

The budding yeast Saccharomyces cerevisiae is a useful system to express recombinant proteins and analyze protein-protein interaction. Membrane-spanning proteins like plant receptor kinases find their way to the plasma membrane when expressed in yeast and seem to retain their structure and function. Here, we describe a general yeast DNA transformation procedure based on lithium acetate, salmon sperm DNA, and polyethylene glycol used to express recombinant proteins. Yeast cells expressing plant receptor kinases can be used for in vivo and in vitro studies of receptor function.

KEYWORDS:

Receptor kinase; Saccharomyces cerevisiae; Transformation; Yeast

PMID:
28567639
DOI:
10.1007/978-1-4939-7063-6_2
[Indexed for MEDLINE]

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