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Magn Reson Chem. 2017 Nov;55(11):996-1005. doi: 10.1002/mrc.4622. Epub 2017 Jun 20.

Practical guide for selection of 1 H qNMR acquisition and processing parameters confirmed by automated spectra evaluation.

Author information

1
Spectral Service AG, Emil-Hoffmann-Straße 33, 50996, Köln, Germany.
2
Institute of Chemistry, Saratov State University, Astrakhanskaya Street 83, 410012, Saratov, Russia.

Abstract

In our recent paper, a new technique for automated spectra integration and quality control of the acquired results in qNMR was developed and validated (Monakhova & Diehl, Magn. Res. Chem. 2017, doi: 10.1002/mrc.4591). The present study is focused on the influence of acquisition and postacquisition parameters on the developed automated routine in particular, and on the quantitative NMR (qNMR) results in general, which has not been undertaken previously in a systematic and automated manner. Results are presented for a number of model mixtures and authentic pharmaceutical products measured on 500- and 600-MHz NMR spectrometers. The influence of the most important acquisition (spectral width, transmitter [frequency] offset, number of scans, and time domain) and processing (size of real spectrum, deconvolution, Gaussian window multiplication, and line broadening) parameters for qNMR was automatically investigated. Moderate modification of the majority of the investigated parameters from default instrument settings within evaluated ranges does not significantly affect the trueness and precision of the qNMR. Lite Gaussian window multiplication resulted in accuracy improvement of the qNMR output and is recommended for routine measurements. In general, given that the acquisition and processing parameters were selected based on the presented guidelines, automated qNMR analysis can be employed for reproducible high-precision concentration measurements in practice.

KEYWORDS:

acquisition; automation; pharmaceutical products; processing; qNMR

PMID:
28561374
DOI:
10.1002/mrc.4622

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