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Sci Adv. 2017 May 19;3(5):e1601910. doi: 10.1126/sciadv.1601910. eCollection 2017 May.

CRISPR/Cas9 gene drives in genetically variable and nonrandomly mating wild populations.

Author information

1
Department of Biology, Indiana University, Bloomington, IN 47405, USA.

Abstract

Synthetic gene drives based on CRISPR/Cas9 have the potential to control, alter, or suppress populations of crop pests and disease vectors, but it is unclear how they will function in wild populations. Using genetic data from four populations of the flour beetle Tribolium castaneum, we show that most populations harbor genetic variants in Cas9 target sites, some of which would render them immune to drive (ITD). We show that even a rare ITD allele can reduce or eliminate the efficacy of a CRISPR/Cas9-based synthetic gene drive. This effect is equivalent to and accentuated by mild inbreeding, which is a characteristic of many disease-vectoring arthropods. We conclude that designing such drives will require characterization of genetic variability and the mating system within and among targeted populations.

KEYWORDS:

CRISPR/Cas9; Population Genetics; gene drive; genetic variation; immune to drive; inbreeding; nonrandom mating; population engineering; population suppression; selfish gene

PMID:
28560324
PMCID:
PMC5438214
DOI:
10.1126/sciadv.1601910
[Indexed for MEDLINE]
Free PMC Article

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