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Sci Rep. 2017 May 30;7(1):2527. doi: 10.1038/s41598-017-02556-9.

Positive regulatory role of sound vibration treatment in Arabidopsis thaliana against Botrytis cinerea infection.

Author information

1
Department of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, 38541, Republic of Korea.
2
Department of Biology, College of Science, United Arab Emirates University, Al Ain, 15551, United Arab Emirates.
3
National Institute of Agricultural Sciences, Rural Development Administration, Wanju, Jeollabuk, 55365, Republic of Korea.
4
Department of Horticulture and Department of Forestry, Michigan State University, East Lansing, MI, 48824-1222, USA.
5
Central Instrument Facility, Gyeongsang National University, Jinju, Gyeongnam, 52828, Republic of Korea.
6
Department of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, 38541, Republic of Korea. hanhongbae@ynu.ac.kr.

Abstract

Sound vibration (SV), a mechanical stimulus, can trigger various molecular and physiological changes in plants like gene expression, hormonal modulation, induced antioxidant activity and calcium spiking. It also alters the seed germination and growth of plants. In this study, we investigated the effects of SV on the resistance of Arabidopsis thaliana against Botrytis cinerea infection. The microarray analysis was performed on infected Arabidopsis plants pre-exposed to SV of 1000 Hertz with 100 decibels. Broadly, the transcriptomic analysis revealed up-regulation of several defense and SA-responsive and/or signaling genes. Quantitative real-time PCR (qRT-PCR) analysis of selected genes also validated the induction of SA-mediated response in the infected Arabidopsis plants pre-exposed to SV. Corroboratively, hormonal analysis identified the increased concentration of salicylic acid (SA) in the SV-treated plants after pathogen inoculation. In contrast, jasmonic acid (JA) level in the SV-treated plants remained stable but lower than control plants during the infection. Based on these findings, we propose that SV treatment invigorates the plant defense system by regulating the SA-mediated priming effect, consequently promoting the SV-induced resistance in Arabidopsis against B. cinerea.

PMID:
28559545
PMCID:
PMC5449397
DOI:
10.1038/s41598-017-02556-9
[Indexed for MEDLINE]
Free PMC Article

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