Format

Send to

Choose Destination
Development. 2017 Jul 1;144(13):2338-2351. doi: 10.1242/dev.143040. Epub 2017 May 30.

Generating minicorneal organoids from human induced pluripotent stem cells.

Author information

1
Sudhakar and Sreekanth Ravi Stem Cell Biology Laboratory, Prof. Brien Holden Eye Research Centre, Hyderabad Eye Research Foundation, L.V. Prasad Eye Institute, Hyderabad 500 034, India.
2
Ophthalmic Pathology Laboratory, L.V. Prasad Eye Institute, Hyderabad 500 034, India.
3
National Centre for Laboratory Animal Sciences, National Institute of Nutrition, Hyderabad 500 007, India.
4
Department of Ophthalmic Plastic and Facial Aesthetic Surgery, L.V. Prasad Eye Institute, Hyderabad 500 034, India.
5
Biochemistry Division, National Institute of Nutrition, Hyderabad 500 007, India.
6
Tej Kohli Cornea Institute, Centre for Ocular Regeneration, L.V. Prasad Eye Institute, Hyderabad 500 034, India.
7
Sudhakar and Sreekanth Ravi Stem Cell Biology Laboratory, Prof. Brien Holden Eye Research Centre, Hyderabad Eye Research Foundation, L.V. Prasad Eye Institute, Hyderabad 500 034, India indumathi@lvpei.org.

Abstract

Corneal epithelial stem cells residing within the annular limbal crypts regulate adult tissue homeostasis. Autologous limbal grafts and tissue-engineered corneal epithelial cell sheets have been widely used in the treatment of various ocular surface defects. In the case of bilateral limbal defects, pluripotent stem cell (PSC)-derived corneal epithelial cells are now being explored as an alternative to allogeneic limbal grafts. Here, we report an efficient method to generate complex three-dimensional corneal organoids from human PSCs. The eye field primordial clusters that emerged from differentiating PSCs developed into whole eyeball-like, self-organized, three-dimensional, miniature structures consisting of retinal primordia, corneal primordia, a primitive eyelid-like outer covering and ciliary margin zone-like adnexal tissues in a stepwise maturation process within 15 weeks. These minicorneal organoids recapitulate the early developmental events in vitro and display similar anatomical features and marker expression profiles to adult corneal tissues. They offer an alternative tissue source for regenerating different layers of the cornea and eliminate the need for complicated cell enrichment procedures.

KEYWORDS:

Corneal organoids; Human induced pluripotent stem cells; Ocular differentiation; Organogenesis

PMID:
28559289
DOI:
10.1242/dev.143040
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center