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J Biol Chem. 2017 Jul 14;292(28):11937-11950. doi: 10.1074/jbc.M117.789164. Epub 2017 May 30.

The UbiK protein is an accessory factor necessary for bacterial ubiquinone (UQ) biosynthesis and forms a complex with the UQ biogenesis factor UbiJ.

Author information

1
From the Aix Marseille Université, CNRS, Laboratoire de Chimie Bactérienne (LCB) UMR 7283, Institut de Microbiologie de la Méditerranée (IMM), 13402, Marseille, France.
2
the Laboratoire de Chimie des Processus Biologiques, UMR8229 CNRS, Collège de France, Université Pierre et Marie Curie, 11 place Marcelin Berthelot, 75 231 Paris Cedex 05, France.
3
the University Grenoble Alpes, Laboratoire Technologies de l'Ingénierie Médicale et de la Complexité-Informatique, Mathématiques et Applications, Techniques de l'Ingénierie Médicale et de la Complexité-Informatique, Mathématiques et Applications, Grenoble (TIMC-IMAG), UMR 5525, 38000 Grenoble, France.
4
the CNRS, TIMC-IMAG, 38000 Grenoble, France.
5
the Departamento de Genética, Universidad de Sevilla, 41012 Sevilla, Spain.
6
the Institut de Biologie Integrative de la cellule, Plateforme Interactions des Macromolécules, I2BC, UMR 9198 CNRS, Bât 430F, 91405 Orsay Cedex, France, and.
7
the Institut de Biologie Integrative de la cellule, Plateforme SICaPS, I2BC, CNRS, Centre de Recherche de Gif, SICaPS, F-91198 Gif-sur-Yvette Cedex, France.
8
the University Grenoble Alpes, Laboratoire Technologies de l'Ingénierie Médicale et de la Complexité-Informatique, Mathématiques et Applications, Techniques de l'Ingénierie Médicale et de la Complexité-Informatique, Mathématiques et Applications, Grenoble (TIMC-IMAG), UMR 5525, 38000 Grenoble, France, fabien.pierrel@univ-grenoble-alpes.fr.
9
the Laboratoire de Chimie des Processus Biologiques, UMR8229 CNRS, Collège de France, Université Pierre et Marie Curie, 11 place Marcelin Berthelot, 75 231 Paris Cedex 05, France, mfontecave@cea.fr.
10
From the Aix Marseille Université, CNRS, Laboratoire de Chimie Bactérienne (LCB) UMR 7283, Institut de Microbiologie de la Méditerranée (IMM), 13402, Marseille, France, barras@imm.cnrs.fr.

Abstract

Ubiquinone (UQ), also referred to as coenzyme Q, is a widespread lipophilic molecule in both prokaryotes and eukaryotes in which it primarily acts as an electron carrier. Eleven proteins are known to participate in UQ biosynthesis in Escherichia coli, and we recently demonstrated that UQ biosynthesis requires additional, nonenzymatic factors, some of which are still unknown. Here, we report on the identification of a bacterial gene, yqiC, which is required for efficient UQ biosynthesis, and which we have renamed ubiK Using several methods, we demonstrated that the UbiK protein forms a complex with the C-terminal part of UbiJ, another UQ biogenesis factor we previously identified. We found that both proteins are likely to contribute to global UQ biosynthesis rather than to a specific biosynthetic step, because both ubiK and ubiJ mutants accumulated octaprenylphenol, an early intermediate of the UQ biosynthetic pathway. Interestingly, we found that both proteins are dispensable for UQ biosynthesis under anaerobiosis, even though they were expressed in the absence of oxygen. We also provide evidence that the UbiK-UbiJ complex interacts with palmitoleic acid, a major lipid in E. coli Last, in Salmonella enterica, ubiK was required for proliferation in macrophages and virulence in mice. We conclude that although the role of the UbiK-UbiJ complex remains unknown, our results support the hypothesis that UbiK is an accessory factor of Ubi enzymes and facilitates UQ biosynthesis by acting as an assembly factor, a targeting factor, or both.

KEYWORDS:

Escherichia coli (E. coli); bioenergetics; coenzyme Q10 (CoQ10); electron transfer; microbiology; ubiquinone

PMID:
28559279
PMCID:
PMC5512085
DOI:
10.1074/jbc.M117.789164
[Indexed for MEDLINE]
Free PMC Article

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