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New Phytol. 2017 Jul;215(2):725-736. doi: 10.1111/nph.14609. Epub 2017 May 30.

A microbially derived tyrosine-sulfated peptide mimics a plant peptide hormone.

Author information

1
Department of Plant Pathology and the Genome Center, University of California, Davis, CA, 95616, USA.
2
Feedstocks Division, Joint BioEnergy Institute and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
3
Department of Plant Biology, Carnegie Institution for Science, Stanford, CA, 94305, USA.
4
Department of Microbiology and Molecular Genetics, University of California, Davis, CA, 95616, USA.

Abstract

The biotrophic pathogen Xanthomonas oryzae pv. oryzae (Xoo) produces a sulfated peptide named RaxX, which shares similarity to peptides in the PSY (plant peptide containing sulfated tyrosine) family. We hypothesize that RaxX mimics the growth-stimulating activity of PSY peptides. Root length was measured in Arabidopsis and rice treated with synthetic RaxX peptides. We also used comparative genomic analyses and reactive oxygen species burst assays to evaluate the activity of RaxX and PSY peptides. Here we found that a synthetic sulfated RaxX derivative comprising 13 residues (RaxX13-sY), highly conserved between RaxX and PSY, induces root growth in Arabidopsis and rice in a manner similar to that triggered by PSY. We identified residues that are required for activation of immunity mediated by the rice XA21 receptor but that are not essential for root growth induced by PSY. Finally, we showed that a Xanthomonas strain lacking raxX is impaired in virulence. These findings suggest that RaxX serves as a molecular mimic of PSY peptides to facilitate Xoo infection and that XA21 has evolved the ability to recognize and respond specifically to the microbial form of the peptide.

KEYWORDS:

PSY1; RaxX; XA21; Xanthomonas oryzae pv. oryzae; molecular mimicry; root growth; tyrosine-sulfated peptide

PMID:
28556915
PMCID:
PMC5901733
DOI:
10.1111/nph.14609
[Indexed for MEDLINE]
Free PMC Article

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