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Biochem Biophys Res Commun. 2017 Jul 22;489(2):206-210. doi: 10.1016/j.bbrc.2017.05.132. Epub 2017 May 26.

Jasmonate-induced biosynthesis of steroidal glycoalkaloids depends on COI1 proteins in tomato.

Author information

1
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama 8916-5, Ikoma, 630-0101, Nara, Japan; Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt. Electronic address: a-aboegala@bs.naist.jp.
2
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama 8916-5, Ikoma, 630-0101, Nara, Japan. Electronic address: chonprakun.thagun@riken.jp.
3
Graduate School of Agricultural Science, Kobe University, Rokkoudai 1-1, Nada, Kobe, 657-8501, Hyogo, Japan. Electronic address: nakayasu@garnet.kobe-u.ac.jp.
4
Graduate School of Agricultural Science, Kobe University, Rokkoudai 1-1, Nada, Kobe, 657-8501, Hyogo, Japan. Electronic address: mizutani@gold.kobe-u.ac.jp.
5
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama 8916-5, Ikoma, 630-0101, Nara, Japan. Electronic address: hasimoto@bs.naist.jp.
6
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama 8916-5, Ikoma, 630-0101, Nara, Japan. Electronic address: t-shouji@bs.naist.jp.

Abstract

In tomato, perception of jasmonates by a receptor complex, which includes the F-box protein CORONATINE INSENSITIVE 1 (COI1), elicits biosynthesis of defensive steroidal glycoalkaloids (SGAs) via a jasmonate-responsive ERF transcription factor, JRE4/GAME9. Although JRE4 is upregulated by jasmonate and induces the expression of many metabolic genes involved in SGA biosynthesis, it is not known whether JRE4 alone is sufficient for increased SGA biosynthesis upon activation of jasmonate signaling. Here, we show that application of methyl jasmonate induces the expression of JRE4 and SGA biosynthesis genes in leaves and hairy roots of wild-type tomato, but not in jasmonic acid insensitive 1 (jai1), a loss-of-function mutant allele of the tomato COI1 gene. Induced overexpression of JRE4 increased the expression of SGA biosynthesis genes in transgenic hairy roots of both wild-type tomato and the jai1 mutant, suggesting that JRE4 is the primary transcription factor that functions downstream of the jasmonate signaling pathway.

KEYWORDS:

COI1; Jasmonate; Steroidal glycoalkaloid; Tomato; α-tomatine

PMID:
28554842
DOI:
10.1016/j.bbrc.2017.05.132
[Indexed for MEDLINE]

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