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Food Chem. 2017 Nov 15;235:294-297. doi: 10.1016/j.foodchem.2017.05.059. Epub 2017 May 13.

Inhibition effect of food preservatives on endoproteinases.

Author information

1
Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 660036 Krasnoyarsk, Russia; Siberian Federal University, Institute of Fundamental Biology and Biotechnology, 660041 Krasnoyarsk, Russia. Electronic address: esimbekova@yandex.ru.
2
Krasnoyarsk State Agricultural University, Institute of Agro-ecological Technologies, 660130 Krasnoyarsk, Russia.
3
Siberian Federal University, Institute of Fundamental Biology and Biotechnology, 660041 Krasnoyarsk, Russia.
4
Siberian Federal University, Institute of Fundamental Biology and Biotechnology, 660041 Krasnoyarsk, Russia; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 660036 Krasnoyarsk, Russia.

Abstract

The present manuscript proposes a novel approach to assess the impact of food additives on human metabolism by analysing their effect on biomarker enzyme activity. Alterations in the activity of pancreatic enzymes, such as chymotrypsin and trypsin, which are affected by the most common food preservatives, sodium benzoate (E211), potassium sorbate (E202) and sorbic acid (E200), have been evaluated. The proteinase activity was analysed with a bioluminescent method using the light intensity decay constant. Our study revealed that the preservatives reduce proteinase activity by 50% (EC50) at a much lower concentration than their acceptable daily intake (ADI). Thus, sodium benzoate and sorbic acid have an inhibition effect on chymotrypsin at concentrations 14 times lower and 70 times lower than their ADI and this increases with exposure time. Food preservative consumption impacts negatively on protein digestion, which is especially dangerous for patients with pancreatitis.

KEYWORDS:

Endoproteinases; Food additives; Pancreatic disease; Pancreatic enzymes; Potassium sorbate E202 (PubChem CID: 23676745); Sodium benzoate E211 (PubChem CID: 517055); Sorbic acid E200 (PubChem CID: 643460)

PMID:
28554639
DOI:
10.1016/j.foodchem.2017.05.059
[Indexed for MEDLINE]

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