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Folia Morphol (Warsz). 2017 May 29. doi: 10.5603/FM.a2017.0037. [Epub ahead of print]

The possible protective effect of L-arginine against 5-fluorouracil-induced nephrotoxicity in male albino rats.

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Al-Azhar University, Egypt.


5-fluorouracil (5-FU) is a potent antineoplastic agent used for the treatment of various malignancies. The L-argininenitric oxide (NO) pathway involved in the pathogenesis of chemotherapy induced kidney damage. This work investigated the beneficial mechanism of L-arginine supplementation in 5 FU induced nephropathy. Eighty male Wistar rats were divided into four equal groups: Control group; L-arginine group (378 mg/rat /day for 4 weeks); 5-FU group (189 mg/rat/week for 4 weeks) and L-arginine for one week before and 4 weeks concomitant with 5-FU group. At the end of experiment, the kidney functions were assessed and kidneys specimens were processed for paraffin sections and stained with H&E, Masson's Trichome and PAS stains. Immunohistochemical demonstration of caspase-3 for apoptosis and inducible nitric oxide synthase (iNOS). Image analyzer was used to analyze the results morphometrically and statisticaly analyzed. L-arginine administration to 5-FU treated animals elicited significant reduction in serum urea and creatinine levels, urine volume, urinary protein excretion and kidney / body weight ratio in comparison to fluorouracil treated group. L-arginine improved glomeruloscelerosis, degeneration of convoluted tubules and interstitial fibrosis in 5-FU treated animals. L-arginine attenuated effectively some biochemical and histological changes in 5-fluorouracil nephrotoxicity.


5-fluorouracil; L-arginine; nephrotoxicity

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