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Anticancer Res. 2017 Jun;37(6):2861-2866.

Protein Complex of Fibrinogen Gamma Chain and Complement Factor H in Ovarian Cancer Patient Plasma.

Author information

1
Department of Obstetrics and Gynecology, Kashiwara Municipal Hospital, Kashiwara, Japan kenhonda@med.osaka-cu.ac.jp.
2
Faculty of Health Science, Butsuryo College of Osaka, Sakai, Japan.
3
NPO Corp. Japanese Center for AntiAging MedSciences, Shobara, Japan.
4
Department of Obstetrics and Gynecology, Osaka City University Graduate School of Medicine, Osaka, Japan.
5
Department of Obstetrics and Gynecology, Kashiwara Municipal Hospital, Kashiwara, Japan.

Abstract

BACKGROUND:

In the plasma of an advanced cancer patient, fibrinogen is sometimes increased with possible effects on red blood cells (RBCs).

MATERIALS AND METHODS:

The plasma fraction deteriorating osmotic resistance of RBCs was separated from a patient's plasma with advanced ovarian cancer by phenyl-sepharose column chromatography and analyzed with gel filtration chromatography.

RESULTS:

In the plasma fraction, we found a protein reactive against whole fibrinogen with a molecular weight higher than that of intact fibrinogen from a healthy volunteer. The-high molecular weight protein was immunoractive to an antibody against fibrinogen gamma chain but not to an antibody against alpha or beta chain. Complement factor H, identified by N-terminal sequencing of a 150-kDa protein separated from the protein, was also eluted from anti-fibrinogen gamma immunoaffinity column.

CONCLUSION:

Fibrinogen gamma chain and complement factor H were found to be bound as a protein complex in the plasma of a patient with advanced ovarian cancer.

KEYWORDS:

Fibrinogen; complement factor H; gamma chain; ovarian cancer

PMID:
28551622
DOI:
10.21873/anticanres.11638
[Indexed for MEDLINE]

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