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Methods Mol Biol. 2018;1577:1-10. doi: 10.1007/7651_2017_34.

Decellularization Methods for Scaffold Fabrication.

Author information

1
Department of Polymer and Process Engineering, Indian Institute of Technology, Roorkee, 247001, India.
2
Department of Chemical Engineering, University of Rhode Island, Kingston, RI, 02881, USA.
3
Department of Polymer and Process Engineering, Indian Institute of Technology, Roorkee, 247001, India. mishrawise@gmail.com.

Abstract

Decellularization is the process of removal of native cells from tissue, leaving behind a three-dimensional (3D) ultrastructure of extracellular matrix (ECM) proteins while preserving the bioactivity and mechanics of the tissue. It offers a unique top-down approach for fabricating ECM based natural scaffold for tissue engineering application. Herein, this chapter presents the fabrication of decellularized scaffold employing different methods: whole organ perfusion, immersion and agitation, pressure gradient, and supercritical fluid. The decellularized scaffold aims to exploit the nature-designed 3D architecture, a successful platform technology, for creating scaffolding materials for tissue engineering and regenerative medicine.

KEYWORDS:

Cells; Decellularization; Extracellular matrix; Scaffold; Tissue engineering

PMID:
28550502
DOI:
10.1007/7651_2017_34
[Indexed for MEDLINE]

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