Format

Send to

Choose Destination
Mol Cell Endocrinol. 2017 Sep 5;452:93-109. doi: 10.1016/j.mce.2017.05.018. Epub 2017 May 23.

Early transcriptome responses of the bovine midcycle corpus luteum to prostaglandin F2α includes cytokine signaling.

Author information

1
Olson Center for Women's Health/Obstetrics and Gynecology Department, University of Nebraska Medical Center, 989450 Nebraska Medical Center, Omaha, NE 68198-9450, USA; Biochemistry and Molecular Biology Department, University of Nebraska Medical Center, 985870 Nebraska Medical Center, Omaha, NE 68198-5870, USA. Electronic address: heather.talbott@unmc.edu.
2
Olson Center for Women's Health/Obstetrics and Gynecology Department, University of Nebraska Medical Center, 989450 Nebraska Medical Center, Omaha, NE 68198-9450, USA. Electronic address: xhou@unmc.edu.
3
Biostatistics Department, University of Nebraska Medical Center, 984375 Nebraska Medical Center, Omaha, NE 68198-4375, USA. Electronic address: fqiu@unmc.edu.
4
Olson Center for Women's Health/Obstetrics and Gynecology Department, University of Nebraska Medical Center, 989450 Nebraska Medical Center, Omaha, NE 68198-9450, USA. Electronic address: pan.zhang@unmc.edu.
5
Department of Genetics, Cell Biology and Anatomy, Bioinformatics and Systems Biology Core, University of Nebraska Medical Center, 985805 Nebraska Medical Center, Omaha, NE 68198-5805, USA. Electronic address: babu.guda@unmc.edu.
6
Biostatistics Department, University of Nebraska Medical Center, 984375 Nebraska Medical Center, Omaha, NE 68198-4375, USA. Electronic address: fangyu@unmc.edu.
7
Nutrition and Environmental Management Research Unit, United States Department of Agriculture, P.O. Box 166 (State Spur 18D)/USDA-ARS-PA-USMARC, Clay Center, NE 68933, USA. Electronic address: bob.cushman@ars.usda.gov.
8
Animal Science Department, University of Nebraska-Lincoln, P.O. Box 830908, C203 ANSC, Lincoln, NE 68583-0908, USA. Electronic address: jwood5@unl.edu.
9
Olson Center for Women's Health/Obstetrics and Gynecology Department, University of Nebraska Medical Center, 989450 Nebraska Medical Center, Omaha, NE 68198-9450, USA. Electronic address: chengwang@unmc.edu.
10
Animal Science Department, University of Nebraska-Lincoln, P.O. Box 830908, C203 ANSC, Lincoln, NE 68583-0908, USA. Electronic address: acupp2@unl.edu.
11
Olson Center for Women's Health/Obstetrics and Gynecology Department, University of Nebraska Medical Center, 989450 Nebraska Medical Center, Omaha, NE 68198-9450, USA; Biochemistry and Molecular Biology Department, University of Nebraska Medical Center, 985870 Nebraska Medical Center, Omaha, NE 68198-5870, USA; Veterans Affairs Medical Center, 4101 Woolworth Ave, Omaha, NE 68105, USA. Electronic address: jsdavis@unmc.edu.

Abstract

In ruminants, prostaglandin F2alpha (PGF2α)-mediated luteolysis is essential prior to estrous cycle resumption, and is a target for improving fertility. To deduce early PGF2α-provoked changes in the corpus luteum a short time-course (0.5-4 h) was performed on cows at midcycle. A microarray-determined transcriptome was established and examined by bioinformatic pathway analysis. Classic PGF2α effects were evident by changes in early response genes (FOS, JUN, ATF3) and prediction of active pathways (PKC, MAPK). Several cytokine transcripts were elevated and NF-κB and STAT activation were predicted by pathway analysis. Self-organizing map analysis grouped differentially expressed transcripts into ten mRNA expression patterns indicative of temporal signaling cascades. Comparison with two analogous datasets revealed a conserved group of 124 transcripts similarly altered by PGF2α treatment, which both, directly and indirectly, indicated cytokine activation. Elevated levels of cytokine transcripts after PGF2α and predicted activation of cytokine pathways implicate inflammatory reactions early in PGF2α-mediated luteolysis.

KEYWORDS:

Corpus luteum; Cytokine; Luteolysis; PGF2α; Regression; Signaling

PMID:
28549990
DOI:
10.1016/j.mce.2017.05.018
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center